Subtype selectivity and functional bias of VPAC receptor positive allosteric modulators (PAMs) for understanding models of pulmonary disease

G protein-coupled receptors are the most important class of receptors in the human genome, are involved in virtually all physiological processes and are key players in major health burdens of the 21st century, including pulmonary diseases. This project seeks to gain a fundamental understanding of the recently emerged biological paradigms of allosteric receptor modulation and ligand-directed signalling bias and the link to physiological outcome. This will be achieved by using the Alchemia proprietary Diversity Scanning Library to create a chemical toolbox of biased ligands that will be used together with optimized biological assays for detecting and quantifying these phenomena.