The goal of the proposal is to establish a single molecule imaging laboratory to close the century-old gap
between structural imaging and cellular imaging. Utilising the expertise of the ARC Centre of Excellence in
Advanced Molecular Imaging, we will design, build and apply three microscopes that go beyond the current
commercial solutions for single molecule localisation microscopy (PALM, STORM) and perform single
molecule imaging (i) deep inside cells and tissue, (ii) with fast acquisition rate to monitor highly dynamic
molecular events, and (iii) improved precision to image molecules and complexes in intact cells with . 1nm
resolution. There is no comparable imaging facility in the world.