Sensing atmosphere: Understanding the HNOX-protein gas-sensing capability and how it is affected by heme-oxidation.

  • Schmidt, Harald (Primary Chief Investigator (PCI))
  • Harper, Barbara (Chief Investigator (CI))
  • Schmidt, Peter (Chief Investigator (CI))
  • Stasch, Johannes-Peter (Chief Investigator (CI))

Project: Research

Project Details

Project Description

The project investigates how HNOX proteins discriminate gaseous ligands, the translation of ligand binding into conformational changes, and how oxidation of the HNOX heme affects these interactions. The project will show by co-crystallization studies how HNOX-binding compounds interact with the HNOX domain to understand how these tools can be used to detect oxidized HNOX domains in general. Various biochemical approaches will be established to quantify and localize oxidized sGC/HNOX domains in living cells. In addition, the project will identify the molecular mechanisms linked to prokaryotic HNOX domains in clostridium strains maybe offering the opportunity to identify novel approaches for the control of pathogen growth.
StatusFinished
Effective start/end date1/01/0831/12/08

Funding

  • Australian Research Council (ARC): AUD93,643.00
  • Australian Research Council (ARC): AUD14,900.00
  • Monash University
  • Australian Research Council (ARC)
  • Australian Research Council (ARC)