Control of poly(A) tail length can affect translation and stability of messenger RNAs. We measured mRNA tail lengths and association with the poly(A)-binding protein Pab1 in the yeast transcriptome and found a wide-spread interdependence between 3' untranslated region-mediated poly(A) tail length control and mRNA translation to co-regulate functionally related mRNAs. Here, we propose to survey mRNA tail length by microarray in a set of cellular conditions to understand why cells employ this mode of adjustable gene control. We further propose a collection of experiments addressing the molecular mechanism to understand how cells organise this previously hidden layer of gene regulation.
|Effective start/end date||2/01/08 → 31/12/12|
- Australian Research Council (ARC): AUD295,929.00