Nuclear plasticity during neutrophil migration and function

Cell migration requires overall cellular plasticity and plasticity of internal structures such as the nucleus. The neutrophil, one of the most peripatetic cell types, has specialised lobulated nucleus, thought to facilitate its mobility and function. However, just how nuclear shape impacts on neutrophil function is largely unexplored. Using new zebrafish reporter lines displaying the nucleus and cytoplasm concurrently, the exceptionally dynamic plasticity of neutrophil nuclei during neutrophil migration and function in vivo can be displayed. This project seeks to use the exceptional spatiotemporal resolution now achievable with a new lattice light sheet microscopy to examine this further, and explore its impact on neutrophil function.