Isolation, processing, expansion, characterization and storage of umbilical cord blood cells and their neurotrophic factors for the treatment of neurological disorders.

Project: Research

Project Description

Research Organisation will investigate the use of umbilical cord blood (UCB) derived stem cells and their derivatives to treat neurological disorders including spinal cord injury, stroke and cerebral palsy. It has been reported that Umbilical Cord Blood derived from term deliveries may contain and secrete neruo-protective and neuro-regenerative factors. However the cell types responsible and the specific factors responsible have not been investigated. The Industry Research Fellow will investigate the mechanism of action underlying the process and ways to expand UCB cells of interest and derivation of neuro-protective and neuro-regenerative factors for potential clinical use. The commercial goal of this project is to produce cells and products that can be used as ‘off the shelf’ treatments for neurological disorders.
The aims of the Project are:
1. To obtain Human Ethics approval for collection of human Umbilical Cord Blood (UCB) from term and preterm deliveries for research purposes.
2. To investigate novel, commercially relevant ways of collection, processing and expansion of UCB.
3. To standardize methods for detection of neurotrophic markers in different stem cell fractions.
4. To characterize the neuro-protective and neuro-regenerative factors produced by UCB derived cells using:
a. Cell surface and intracellular markers using microchip based flow cytometry
b. Secretory proteins using multiplexing technology
c. Genome transcriptome analysis, followed by bioinformatics network and pathway analysis
5. To define which UCB derived cell types are responsible for neurotrophic effects of UCB derived cells.
6. To develop methods isolate and expand specific cell types within UCB that produce neuro –protective and neuro-regenerative via the study of:
a. Specific cell fractions
b. Time in culture
c. Expression of neurotrophic factors by specific UCB cell types
d. Impact of physiological culture variables
e. Impact of addition of growth factors
StatusFinished
Effective start/end date31/07/1630/06/19

Keywords

  • Stem cells
  • Regenerative medicine
  • Ubilical cord blood
  • Neurotrophic factors