Biodiversity faces an unpredictable complex of human impacts and global environmental change. The best insurance for biodiversity in changing and uncertain environments is genetic functional variability and flexibility. Accordingly, the main aim of conservation is to maintain ecological diversity and evolutionary potential, but this has been prohibitively difficult to assess. Now the genomics revolution provides the tools needed. We have assembled an outstanding multidisciplinary team to apply genomics to a suite of Australian native freshwater fish to address the range of typical management needs. Working with key freshwater management agencies, we will prove the technologies in an array of current pressing management situations.