Functional Studies of Tyrosine Sulfation using Synthetic Sulfoproteins

This project aims to address a lack of knowledge about how post-translational sulfation of tyrosine residues influences protein function. The project will develop a much needed synthetic platform for the rapid and efficient generation of libraries of site-selectively sulfated proteins. Underpinned by the ability to access synthetic sulfoproteins, expected outcomes include a detailed understanding of how tyrosine sulfation can modulate function and stability of antibodies and proteins with anticoagulant and anti-inflammatory activities. Significant benefits of the project will include breakthrough technologies for the preparation of homogeneously modified proteins, which will strengthen Australia’s growing biotechnology sector.