Functional characterisation of neurons derived from embryonic stem cells and NS cells

Embryonic stem cells can be used as a source of neural stem cells (NS cells) which can be stabilised in a chemically defined growth medium, expanded and cryopreserved. NS cells represent a new and renewable source of brain cells (neurons, astrocytes, and oligodendrocytes) which will have many important applications in research and therapy. As yet the properties of cells derived from NS cells have not been investigated. The project will examine NS cells as a source of defined subtypes of neurons, establish appropriate new culture methods, and will investigate the biological function of these neurons using cell physiological and pharmacological techniques.