Allosteric Fingerprinting of G Protein-Coupled Receptor Monomers and Oligomers

Project: Research

Project Description

Proposed research will exploit the exquisite sensitivity of allosteric interactions to conformational changes, to develop an enabling technology that can unambiguously fingerprint endogenously expressed G protein-coupled receptor (GPCR) complexes. The hypothesis is that GPCR monomers, dimers and higher order oligomers will have a unique profile of allosteric modulation, which in turn can be used to probe for GPCR interactions within healthy and disease tissue. The specific aims are: 1.To establish how the nature of the GPCR complex influences allosterism through systematic analysis of intramolecular and intermolecular allosteric interactions. 2.To fingerprint GPCR complexes within endogenous expression systems using allosteric profiling.
StatusFinished
Effective start/end date1/01/1316/11/16

Funding

  • Australian Research Council (ARC): AUD255,000.00
  • Australian Research Council (ARC): AUD120,000.00
  • Australian Research Council (ARC)