Electron microscopes are key tools for selectively analysing nanostructures, from individual nanoparticles to embedded precipitates. However, the important properties of nanostructured materials are normally measured at the macroscopic level, not at the level of the nanostructure. This Facility will provide a powerful new capability to Australia, enabling the measurement and correlation of structure and properties at the nanoscale and in real time. It will be integrated across Victoria's three largest electron microscope facilities providing comprehensive in-situ electrical, mechanical and thermal measurements. These capabilities are essential to advance a large range of multidisciplinary research projects at the cutting-edge of science.