The proposed cryo-Focused Ion Beam Scanning Electron Microscope (Cryo-FIBSEM) will reveal isometric
3D information on the structure and composition of specimens at the nanometer scale. The cryo-FIBSEM will
be the first instrument of this type in Australia able to operate in a low temperature cryogenic mode. This will
enable the imaging of vitrified biological materials in a near native state and of non-biological material to allow imaging of for example fluids, emulsions, gels and interfaces between biological and non-biological materials. Synergistic workflows incorporating unique high-end microscopes at Monash will enable studying complex biological structures in their native context and atomic scale imaging of beam sensitive materials.