Broadly my research brings together biology and engineering to address sustainable manufacturing.

I am interested in discovering and designing enzymes for specific and fast reactions and applied as biocatalysts for use in vitro and in vivo. Also I research the metabolic and systems biology of yeast and bacterial cells in culture, examining the exometabolome and recombinant production of proteins and investigating heterogeneity among cells using our high throughput culture and single cell analysis facility.

I use biocatalysts at different scales; the simplest scale being the isolated enzyme to the most complex such as a self-replicating cell. The easiest to implement is an isolated enzymes (biocatalysts) for application in cellulose and food processing through to cascade/tandem reaction systems. At the other extreme, whole cells have complex biochemical pathways to consider and require approaches like synthetic biology to streamline and improve production of selected metabolites.

Also, I have an active research program in food processing supported by industry projects with particular interest in polyphenol antioxidants, dietary fibres and valorising food byproducts.

For more information go to my group site at https://sites.google.com/monash.edu/haritos-group/home

Enzyme discovery and design; synthetic biology of yeast and bacteria for bioproduction; food byproduct valorisation

Application areas: biocatalysis; bioprocessing; fermentation

• CHE5322 Advanced Biochemical Technology
• CHE5321 Advanced Bioprocess Engineering
• ENG4701 and ENG4702 Research Project