Sebastian Furness

Dr

Accepting PhD Students

PhD projects

1. Single molecule studies on receptor - G protein coupling 2. Molecular basis of cholesterol sensitivity of the cholecystokinin receptor 3. Molecular basis for G protein pleiotropy at the parathyroid hormone receptor

20062020

Research output per year

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Research Output

2020

Activation of the GLP-1 receptor by a non-peptidic agonist

Zhao, P., Liang, Y. L., Belousoff, M. J., Deganutti, G., Fletcher, M. M., Willard, F. S., Bell, M. G., Christe, M. E., Sloop, K. W., Inoue, A., Truong, T. T., Clydesdale, L., Furness, S. G. B., Christopoulos, A., Wang, M. W., Miller, L. J., Reynolds, C. A., Danev, R., Sexton, P. M. & Wootten, D., 8 Jan 2020, In : Nature. 577, 7790, p. 432-436 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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11 Citations (Scopus)
2019

Conformational Transitions and the Activation of Heterotrimeric G Proteins by G Protein-Coupled Receptors

Draper-Joyce, C. & Furness, S. G. B., 26 Jul 2019, In : ACS Pharmacology & Translational Science. 2, 4, p. 285-290 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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Deconvoluting the Molecular Control of Binding and Signaling at the Amylin 3 Receptor: RAMP3 Alters Signal Propagation through Extracellular Loops of the Calcitonin Receptor

Pham, V., Zhu, Y., Dal Maso, E., Reynolds, C. A., Deganutti, G., Atanasio, S., Hick, C. A., Yang, D., Christopoulos, A., Hay, D. L., Furness, S. G. B., Wang, M-W., Wootten, D. & Sexton, P. M., 18 Mar 2019, In : ACS Pharmacology & Translational Science. 2, 3, p. 183-197 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas

Ostrovskaya, A., Hick, C., Hutchinson, D. S., Stringer, B. W., Wookey, P. J., Wootten, D., Sexton, P. M. & Furness, S. G. B., 18 Feb 2019, In : BMC Cancer. 19, 1, 12 p., 157.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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1 Citation (Scopus)

The Molecular Control of Calcitonin Receptor Signaling

dal Maso, E., Glukhova, A., Zhu, Y., Garcia-Nafria, J., Tate, C. G., Atanasio, S., Reynolds, C. A., Ramirez-Aportela, E., Carazo, J-M., Hick, C. A., Furness, S. G. B., Hay, D. L., Liang, Y-L., Miller, L. J., Christopoulos, A., Wang, M-W., Wootten, D. & Sexton, P. M., 11 Jan 2019, In : ACS Pharmacology & Translational Science. 2, 1, p. 31-51 21 p.

Research output: Contribution to journalArticleResearchpeer-review

The nature of efficacy at G protein-coupled receptors

Zhao, P. & Furness, S. G. B., 1 Dec 2019, In : Biochemical Pharmacology. 170, 113647.

Research output: Contribution to journalReview ArticleResearchpeer-review

2 Citations (Scopus)

TrkB Agonist LM22A-4 Increases Oligodendroglial Populations During Myelin Repair in the Corpus Callosum

Nguyen, H. T. H., Wood, R. J., Prawdiuk, A. R., Furness, S. G. B., Xiao, J., Murray, S. S. & Fletcher, J. L., 27 Aug 2019, In : Frontiers in Molecular Neuroscience. 12, 12 p., 205.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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1 Citation (Scopus)
2018

Characterization of signalling and regulation of common calcitonin receptor splice variants and polymorphisms

Dal Maso, E., Just, R., Hick, C., Christopoulos, A., Sexton, P. M., Wootten, D. & Furness, S. G. B., 1 Feb 2018, In : Biochemical Pharmacology. 148, p. 111-129 19 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)
4 Citations (Scopus)

Dominant Negative G Proteins Enhance Formation and Purification of Agonist-GPCR-G Protein Complexes for Structure Determination

Liang, Y-L., Zhao, P., Draper-Joyce, C. J., Baltos, J-A., Glukhova, A., Truong, T. T., May, L. T., Christopoulos, A., Wootten, D., Sexton, P. & Furness, S., 2018, In : ACS Pharmacology & Translational Science. 1, 1, p. 12-20 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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Extracellular loops 2 and 3 of the calcitonin receptor selectively modify agonist binding and efficacy

Dal Maso, E., Zhu, Y., Pham, V., Reynolds, C. A., Deganutti, G., Hick, C. A., Yang, D., Christopoulos, A., Hay, D. L., Wang, M. W., Sexton, P. M., Furness, S. G. B. & Wootten, D., 1 Apr 2018, In : Biochemical Pharmacology. 150, p. 214-244 31 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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15 Citations (Scopus)

Phase-plate cryo-EM structure of a biased agonistbound human GLP-1 receptor-Gs complex

Liang, Y. L., Khoshouei, M., Glukhova, A., Furness, S. G. B., Zhao, P., Clydesdale, L., Koole, C., Truong, T. T., Thal, D. M., Lei, S., Radjainia, M., Danev, R., Baumeister, W., Wang, M. W., Miller, L. J., Christopoulos, A., Sexton, P. M. & Wootten, D., 1 Mar 2018, In : Nature. 555, 7694, p. 121-125 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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115 Citations (Scopus)

Structure of the adenosine-bound human adenosine A1 receptor-Gi complex

Draper-Joyce, C. J., Khoshouei, M., Thal, D. M., Liang, Y. L., Nguyen, A. T. N., Furness, S. G. B., Venugopal, H., Baltos, J. A., Plitzko, J. M., Danev, R., Baumeister, W., May, L. T., Wootten, D., Sexton, P. M., Glukhova, A. & Christopoulos, A., 20 Jun 2018, In : Nature. 558, 7711, p. 559-565 7 p.

Research output: Contribution to journalArticleResearchpeer-review

100 Citations (Scopus)
2017

Coding GPCR-G protein specificity

Furness, S. G. B. & Sexton, P. M., 1 Oct 2017, In : Cell Research. 27, 10, p. 1193-1194 2 p.

Research output: Contribution to journalShort SurveyResearchpeer-review

Open Access
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1 Citation (Scopus)

Dianthin-30 or gelonin versus monomethyl auristatin E, each configured with an anti-calcitonin receptor antibody, are differentially potent in vitro in high-grade glioma cell lines derived from glioblastoma

Gilabert-Oriol, R., Furness, S. G. B., Stringer, B. W., Weng, A., Fuchs, H., Day, B. W., Kourakis, A., Boyd, A. W., Hare, D. L., Thakur, M., Johns, T. G. & Wookey, P. J., 2017, In : Cancer Immunology Immunotherapy. 66, 9, p. 1217–1228 12 p.

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Phase-plate cryo-EM structure of a class B GPCR-G-protein complex

Liang, Y-L., Khoshouei, M., Radjainia, M., Zhang, Y., Glukhova, A., Tarrasch, J., Thal, D., Furness, S. G. B., Christopoulos, G., Coudrat, T., Danev, R., Baumeister, W., Miller, L. J., Christopoulos, A., Kobilka, B., Wootten, D., Skiniotis, G. & Sexton, P., 1 Jun 2017, In : Nature. 546, 7656, p. 118-123 6 p.

Research output: Contribution to journalArticleResearchpeer-review

237 Citations (Scopus)

What determines the magnitude of cellular response for activation of G protein-coupled receptors?

Furness, S. G. B., Wootten, D. & Sexton, P. M., 6 Feb 2017, In : Cell Cycle. 16, 5, p. 392-394 3 p.

Research output: Contribution to journalEditorialOtherpeer-review

2016

A hydrogen-bonded polar network in the core of the glucagon-like peptide-1 receptor is a fulcrum for biased agonism: lessons from class B crystal structuress

Wootten, D., Reynolds, C. A., Koole, C., Smith, K. J., Mobarec, J. C., Simms, J., Quon, T., Coudrat, T., Furness, S. G. B., Miller, L. J., Christopoulos, A. & Sexton, P. M., 1 Mar 2016, In : Molecular Pharmacology. 89, 3, p. 335-347 13 p.

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

A novel ligand of calcitonin receptor reveals a potential new sensor that modulates programmed cell death

Furness, S. G. B., Hare, D. L., Kourakis, A., Turnley, A. M. & Wookey, P. J., 12 Dec 2016, In : Cell Death Discovery. 2, 1, 9 p., 16062.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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3 Citations (Scopus)

Calcitonin

Ostrovskaya, A., Findlay, D., Sexton, P. & Furness, S., 1 Jan 2016, Reference Module in Neuroscience and Biobehavioral Psychology. Elsevier, 12 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

3 Citations (Scopus)
22 Citations (Scopus)

Ligand-dependent modulation of G protein conformation alters drug efficacy

Furness, S. G. B., Liang, Y-L., Nowell, C. J., Halls, M. L., Wookey, P. J., Dal Maso, E., Inoue, A., Christopoulos, A., Wootten, D. & Sexton, P. M., 20 Oct 2016, In : Cell. 167, 3, p. 739-749 11 p.

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

The extracellular surface of the GLP-1 receptor is a molecular trigger for biased agonism

Wootten, D., Reynolds, C. A., Smith, K. J., Mobarec, J. C., Koole, C., Savage, E. E., Pabreja, K., Simms, J., Sridhar, R., Furness, S. G. B., Liu, M., Thompson, P. E., Miller, L. J., Christopoulos, A. & Sexton, P. M., 16 Jun 2016, In : Cell. 165, 7, p. 1632-1643 12 p.

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)
2015

Biased allosteric modulation at the CaS receptor engendered by structurally diverse calcimimetics

Cook, A. E., Mistry, S. N., Gregory, K. J., Furness, S. G. B., Sexton, P., Scammells, P. J., Conigrave, A., Christopoulos, A. & Leach, K., 2015, In : British Journal of Pharmacology. 172, 1, p. 185 - 200 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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45 Citations (Scopus)
2014

Molecular mechanisms underlying physiological and receptor pleiotropic effects mediated by GLP-1R activation

Pabreja, K., Mohd, M., Koole, C. R., Wootten, D. L. & Furness, S. G. B., 2014, In : British Journal of Pharmacology. 171, 5, p. 1114 - 1128 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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40 Citations (Scopus)

Prolonged calcitonin receptor signaling by salmon, but not human calcitonin, reveals ligand bias

Andreassen, K. V., Hjuler, S. T., Furness, S. G. B., Sexton, P. M., Christopoulos, A., Nosjean, O., Karsdal, M. A. & Henriksen, K., 2014, In : PLoS ONE. 9, 3 (Art. No: e92042), p. 1 - 10 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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35 Citations (Scopus)
2013

A simple method to generate stable cell lines for the analysis of transient protein-protein interactions

Savage, E. E., Wootten, D. L., Christopoulos, A., Sexton, P. & Furness, S. G. B., 2013, In : BioTechniques. 54, 4, p. 217 - 221 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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11 Citations (Scopus)

Recent advances in understanding GLP-1R (glucagon-like peptide-1 receptor) function

Koole, C. R., Pabreja, K., Savage, E. E., Wootten, D. L., Furness, S. G. B., Miller, L. J., Christopoulos, A. & Sexton, P., 2013, In : Biochemical Society Transactions. 41, 1, p. 172 - 179 8 p.

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)
2012

Consequences of splice variation on secretin family G protein-coupled receptor function

Furness, S. G. B., Wootten, D. L., Christopoulos, A. & Sexton, P. M., 2012, In : British Journal of Pharmacology. 166, 1, p. 98 - 109 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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29 Citations (Scopus)

Glucagon-like peptide-1 receptor dimerization differentially regulates agonist signaling but does not affect small molecule allostery

Harikumar, K. G., Wootten, D. L., Pinon, D. I., Koole, C. R., Ball, A. M., Furness, S. GB., Graham, B., Dong, M., Christopoulos, A., Miller, L. J. & Sexton, P. M., 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 45, p. 18607 - 18612 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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43 Citations (Scopus)

Interaction with caveolin-1 modulates G protein coupling of the mouse beta3-adrenoceptor

Sato, M., Hutchinson, D. S., Halls, M. L., Furness, S. G. B., Bengtsson, T., Evans, B. A. & Summers, R. J., 2012, In : The Journal of Biological Chemistry. 287, 24, p. 20674 - 20688 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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19 Citations (Scopus)

Small molecule allosteric modulation of the glucagon-like Peptide-1 receptor enhances the insulinotropic effect of oxyntomodulin

Willard, F. S., Wootten, D. L., Showalter, A. D., Savage, E. E., Ficorilli, J., Farb, T. B., Bokvist, K., Alsina-Fernandez, J., Furness, S. GB., Christopoulos, A., Sexton, P. M. & Sloop, K. W., 2012, In : Molecular Pharmacology. 82, 6, p. 1066 - 1073 8 p.

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172

Wookey, P. J., McLean, C. A., Hwang, P. Y. K., Furness, S. G. B., Nguyen, S., Kourakis, A., Hare, D. L. & Rosenfeld, J. V., 2012, In : Histopathology. 60, 6, p. 895 - 910 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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12 Citations (Scopus)

Xenobiotics and loss of cell adhesion drive distinct transcriptional outcomes by aryl hydrocarbon receptor signaling

Hao, N., Lee, K. L., Furness, S. G. B., Bosdotter, C., Poellinger, L. & Whitelaw, M. L., 2012, In : Molecular Pharmacology. 82, 6, p. 1082 - 1093 12 p.

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)
2010

Amino acid substitutions in the aryl hydrocarbon receptor ligand binding domain reveal YH439 as an atypical AhR activator

Whelan, F., Hao, N., Furness, S. GB., Whitelaw, M. L. & Chapman-Smith, A., 2010, In : Molecular Pharmacology. 77, 6, p. 1037 - 1046 10 p.

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Understanding amylin Receptors

Just, R., Simms, J., Furness, S. G. B., Christopoulos, A. & Sexton, P. M., 2010, The Calcitonin Gene-related Peptide Family: Form, Function and Future Perspectives. Springer, p. 41-57 17 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

2 Citations (Scopus)
2009

Sulfonation and phosphorylation of regions of the dioxin receptor susceptible to methionine modifications.

Dave, K. A., Whelan, F., Bindloss, C., Furness, S. G., Chapman-Smith, A., Whitelaw, M. L. & Gorman, J. J., 2009, In : Molecular & Cellular Proteomics. 8, 4, p. 706 - 719 14 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

The pleiotropy of dioxin toxicity - Xenobiotic misappropriation of the aryl hydrocarbon receptor's alternative physiological roles

Furness, S. & Whelan, F., 2009, In : Pharmacology and Therapeutics. 124, 3, p. 336 - 353 18 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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49 Citations (Scopus)
2008

Receptor activity-modifying proteins differentially modulate the G protein-coupling efficiency of amylin receptors

Morfis, M., Tilakaratne, N., Furness, S. G. B., Christopoulos, G., Werry, T. D., Christopoulos, A. & Sexton, P., 2008, In : Endocrinology. 149, 11, p. 5423 - 5431 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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86 Citations (Scopus)
2007

Identification of N,Nepsilon-dimethyl-lysine in the murine dioxin receptor using MALDI-TOF/TOF- and ESI-LTQ-Orbitrap-FT-MS

Dave, K. A., Hamilton, B. R., Wallis, T. P., Furness, S. G. B., Whitelaw, M. L. & Gorman, J. J., 2007, In : International Journal of Mass Spectrometry. 268, 2-3, p. 168 - 180 13 p.

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

The dioxin (aryl hydrocarbon) receptor as a model for adaptive responses of bHLH/PAS transcription factors

Furness, S. G. B., Lees, M. J. & Whitelaw, M. L., 2007, In : FEBS Letters. 581, 19, p. 3616 - 3625 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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63 Citations (Scopus)
2006

Beyond mere markers: Functions for CD34 family of sialomucins in hematopoiesis

Furness, S. G. B. & McNagny, K., 2006, In : Immunologic Research. 34, 1, p. 13 - 32 20 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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88 Citations (Scopus)

Na+/H+ exchanger regulatory factor-1 is a hematopoietic ligand for a subset of the CD34 family of stem cell surface proteins

Tan, P. C., Furness, S. G. B., Merkens, H., Lin, S., McCoy, M. L., Roskelley, C. D., Kast, J. & McNagny, K. M., 2006, In : Stem Cells. 24, 5, p. 1150 - 1161 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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20 Citations (Scopus)