1989 …2019

Research output per year

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Research Output

1989

A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L

Pike, R. & Dennison, C., 1 Jan 1989, In : Preparative Biochemistry. 19, 3, p. 231-245 15 p.

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate

Jacobs, G. R., Pike, R. N. & Dennison, C., 1 Jan 1989, In : Analytical Biochemistry. 180, 1, p. 169-171 3 p.

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Protein fractionation by three phase partitioning (TPP) in aqueous/t‐butanol mixtures

Pike, R. N. & Dennison, C., 1 Jan 1989, In : Biotechnology and Bioengineering. 33, 2, p. 221-228 8 p.

Research output: Contribution to journalLetterOtherpeer-review

80 Citations (Scopus)
1991

Anti-peptide antibodies to cathepsins B, L and D and type IV collagenase. Specific recognition and inhibition of the enzymes

Coetzer, T. H. T., Elliott, E., Fortgens, P. H., Pike, R. N. & Dennison, C., 15 Feb 1991, In : Journal of Immunological Methods. 136, 2, p. 199-210 12 p.

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

A peptide antibody that specifically inhibits cathepsin L

Dennison, C. & Pike, R. N., 1 Dec 1991, In : Advances in Experimental Medicine and Biology. 303, p. 285-288 4 p.

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)
1992

Characterisation of the Activity and Stability of Single-chain Cathepsin L and of Proteolytically Active Cathepsin L/Cystatin Complexes

Dennison, C., Pike, R., Coetzer, T. & Kirk, K., 1 Jan 1992, In : Biological Chemistry Hoppe-Seyler. 373, 2, p. 419-426 8 p.

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Localization of an immunoinhibitory epitope of the cysteine proteinase, cathepsin l

Coetzer, T. H. T., Pike, R. N. & Dennison, C., 1 Jan 1992, In : Immunological Investigations. 21, 6, p. 495-506 12 p.

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Proteolytically active complexes of cathepsin L and a cysteine proteinase inhibitor; purification and demonstration of their formation in vitro

Pike, R. N., Coetzer, T. H. T. & Dennison, C., 1 May 1992, In : Archives of Biochemistry and Biophysics. 294, 2, p. 623-629 7 p.

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)
1994

Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins

Pike, R., McGraw, W., Potempa, J. & Travis, J., 1 Jan 1994, In : The Journal of Biological Chemistry. 269, 1, p. 406-411 6 p.

Research output: Contribution to journalArticleResearchpeer-review

296 Citations (Scopus)
151 Citations (Scopus)

The role of proteolytic enzymes in the development of pulmonary emphysema and periodontal disease

Travis, J., Pike, R., Imamura, T. & Potempa, J., 1 Dec 1994, In : American Journal of Respiratory and Critical Care Medicine. 150, 6 II, p. S143-S146 4 p.

Research output: Contribution to journalConference articleResearchpeer-review

51 Citations (Scopus)
1995

Baboon (Papio ursinus) cathepsin L: purification, characterization and comparison with human and sheep cathepsin L

Coetzer, T. H. T., Dennehy, K. M., Pike, R. N. & Dennison, C., 1 Jan 1995, In : Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 112, 3, p. 429-439 11 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Dependence of vascular permeability enhancement on cysteine proteinases in vesicles of Porphyromonas gingivalis

Imamura, T., Potempa, J., Pike, R. N. & Travis, J., 1 Jan 1995, In : Infection and Immunity. 63, 5, p. 1999-2003 5 p.

Research output: Contribution to journalArticleResearchpeer-review

82 Citations (Scopus)

Effect of free and vesicle-bound cysteine proteinases of Porphyromonas gingivalis on plasma clot formation: Implications for bleeding tendency at periodontitis sites

Imamura, T., Potempa, J., Pike, R. N., Moore, J. N., Barton, M. H. & Travis, J., 1 Jan 1995, In : Infection and Immunity. 63, 12, p. 4877-4882 6 p.

Research output: Contribution to journalArticleResearchpeer-review

88 Citations (Scopus)

Host and Porphyromonas gingivalis proteinases in periodontitis: A biochemical model of infection and tissue destruction

Potempa, J., Pike, R. & Travis, J., 1 Jul 1995, In : Perspectives in Drug Discovery and Design. 2, 3, p. 445-458 14 p.

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Mature cathepsin L is substantially active in the ionic milieu of the extracellular medium

Dehrmann, F. M., Coetzer, T. H. T., Pike, R. N. & Dennison, C., 1 Jan 1995, In : Archives of Biochemistry and Biophysics. 324, 1, p. 93-98 6 p.

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Molecular cloning and structural characterization of the Arg-gingipain proteinase of Porphyromonas gingivalis: Biosynthesis as a proteinase-adhesin polyprotein

Pavloff, N., Potempa, J., Pike, R. N., Prochazka, V., Kiefer, M. C., Travis, J. & Barr, P. J., 20 Jan 1995, In : The Journal of Biological Chemistry. 270, 3, p. 1007-1010 4 p.

Research output: Contribution to journalArticleResearchpeer-review

163 Citations (Scopus)

The multiple forms of trypsin-like activity present in various strains of Porphyromonas gingivalis are due to the presence of either Arg-gingipain or Lys-gingipain

Potempa, J., Pike, R. & Travis, J., 1 Jan 1995, In : Infection and Immunity. 63, 4, p. 1176-1182 7 p.

Research output: Contribution to journalArticleResearchpeer-review

207 Citations (Scopus)
1996

Characterization of the binding activities of proteinase-adhesin complexes from Porphyromonas gingivalis

Pike, R. N., Potempa, J., Mcgraw, W., Coetzer, T. H. T. & Travis, J., 1 Jan 1996, In : Journal of Bacteriology. 178, 10, p. 2876-2882 7 p.

Research output: Contribution to journalArticleResearchpeer-review

124 Citations (Scopus)

Cleavage of the Human C5a Receptor by Proteinases Derived from Porphyromonas gingivalis: Cleavage of Leukocyte C5a Receptor

Jagels, M. A., Ember, J. A., Travis, J., Potempa, J., Pike, R. & Hugli, T. E., 1 Dec 1996, In : Advances in Experimental Medicine and Biology. 389, p. 155-164 10 p.

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)

Proteases from Trypanosoma brucei brucei: Purification, characterisation and interactions with host regulatory molecules

Troeberg, L., Pike, R. N., Morty, R. E., Berry, R. K., Coetzer, T. H. T. & Lonsdale-Eccles, J. D., 1 Jan 1996, In : European Journal of Biochemistry. 238, 3, p. 728-736 9 p.

Research output: Contribution to journalArticleResearchpeer-review

79 Citations (Scopus)

Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis

Jagels, M. A., Travis, J., Potempa, J., Pike, R. & Hugli, T. E., 1 Jun 1996, In : Infection and Immunity. 64, 6, p. 1984-1991 8 p.

Research output: Contribution to journalArticleResearchpeer-review

88 Citations (Scopus)

Purification and characterization of a novel endopeptidase in ragweed (Ambrosia artemisiifolia) pollen

Bagarozzi, D. A., Pike, R., Potempa, J. & Travist, J., 29 Oct 1996, In : The Journal of Biological Chemistry. 271, 42, p. 26227-26232 6 p.

Research output: Contribution to journalArticleResearchpeer-review

39 Citations (Scopus)

The amino acid sequences, structure comparisons and inhibition kinetics of sheep cathepsin L and sheep stefin B

Ritonja, A., Coetzer, T. H. T., Pike, R. N. & Dennison, C., 1 Jan 1996, In : Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 114, 2, p. 193-198 6 p.

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)
1997

Immunological Cross-Reactivity of the Major Allergen from Perennial Ryegrass (Lolium perenne), Lol p I, and the Cysteine Proteinase, Bromelain

Pike, R. N., Bagarozzi, D. & Travis, J., 1 Jan 1997, In : International Archives of Allergy and Immunology. 112, 4, p. 412-414 3 p.

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Mechanisms of antithrombin polymerisation and heparin activation probed by the insertion of synthetic reactive loop peptides

Fitton, H. L., Pike, R. N., Carrell, R. W. & Chang, W. S. W., 1 Sep 1997, In : Biological Chemistry. 378, 9, p. 1059-1063 5 p.

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Molecular cloning and characterization of Porphyromonas gingivalis lysine-specific gingipain: A new member of an emerging family of pathogenic bacterial cysteine proteinases

Pavloff, N., Pemberton, P. A., Potempa, J., Chen, W. C. A., Pike, R. N., Prochazka, V., Kiefer, M. C., Travis, J. & Barr, P. J., 1 Jan 1997, In : The Journal of Biological Chemistry. 272, 3, p. 1595-1600 6 p.

Research output: Contribution to journalArticleResearchpeer-review

109 Citations (Scopus)

Porphyromonas gingivalis proteinases as virulence factors in the development of periodontitis

Travis, J., Pike, R., Imamura, T. & Potempa, J., 1 Jan 1997, In : Journal of Periodontal Research. 32, 1 PART 2, p. 120-125 6 p.

Research output: Contribution to journalArticleResearchpeer-review

118 Citations (Scopus)

Production of anti-peptide antibodies against trypanopain-Tb from Trypanosoma brucei brucei: Effects of antibodies on enzyme activity against Z-Phe-Arg-AMC

Troeberg, L., Pike, R. N., Lonsdale-Eccles, J. D. & Coetzer, T. H. T., 1 Jun 1997, In : Immunopharmacology. 36, 2-3, p. 295-303 9 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

The anticoagulant activation of antithrombin by heparin

Jin, L., Abrahams, J. P., Skinner, R., Petitou, M., Pike, R. N. & Carrell, R. W., 23 Dec 1997, In : Proceedings of the National Academy of Sciences of the United States of America. 94, 26, p. 14683-14688 6 p.

Research output: Contribution to journalArticleResearchpeer-review

586 Citations (Scopus)

The potential role of α2-macroglobulin in the control of cysteine proteinases (gingipains) from Porphyromonas gingivalis

Grøn, H., Pike, R., Potempa, J., Travis, J., Thøgersen, I. B., Enghild, J. J. & Pizzo, S. V., 1 Jan 1997, In : Journal of Periodontal Research. 32, 1 PART 1, p. 61-68 8 p.

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Titration and Mapping of the Active Site of Cysteine Proteinases from Porphyromonas gingivalis (Gingipains) Using Peptidyl Chloromethanes

Potempa, J., Pike, R. & Travis, J., 1 Jan 1997, In : Biological Chemistry. 378, 3-4, p. 223-230 8 p.

Research output: Contribution to journalArticleResearchpeer-review

134 Citations (Scopus)
1998

Activation ofproteinase activated receptor - 2 on human neutrophils by arginine gingpain from Porphyromonas gingivalis

Lourbakos, A., Chinni, C., Mackie, E. J. & Pike, R. N., 1998, p. 51 - 51. 1 p.

Research output: Contribution to conferenceAbstract

Angiotensinogen cleavage by renin: importance of a structurally constrained N-terminus

Streatfeild-James, R. M., Williamson, D., Pike, R. N., Tewksbury, D., Carrell, R. W. & Coughlin, P. B., 1998, In : FEBS Letters. p. 267 - 270 4 p.

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)

Antithrombins Wibble and Wobble (T85M/K): Archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation

Beauchamp, N. J., Pike, R. N., Daly, M., Butler, L., Makris, M., Dafforn, T. R., Zhou, A., Fitton, H. L., Preston, F. E., Peake, I. R. & Carrell, R. W., 15 Oct 1998, In : Blood. 92, 8, p. 2696-2706 11 p.

Research output: Contribution to journalArticleResearchpeer-review

92 Citations (Scopus)

A trypanosome oligopeptidase as a target for the trypanocidal agents pentamidine, diminazene and suramin

Morty, R. E., Troeberg, L., Pike, R. N., Jones, R., Nickel, P., Lonsdale-Eccles, J. D. & Coetzer, T. H., 1998, In : FEBS Letters. p. 251 - 256 6 p.

Research output: Contribution to journalArticleResearchpeer-review

61 Citations (Scopus)

Characterization of a recombinant Fasciola Hepatica cathepsin L

Pike, R. N., Smooker, P. M., Irving, J., Whisstock, J. C. & Spithill, T. W., 1998, p. 63 - 63. 1 p.

Research output: Contribution to conferenceAbstract

Cleavage and activation of preinase-activated receptor-2 on human neutrphil s by gingpain -R from Porphyromonas gingivilis

Lourbakos, A., Chinni, C., Thompson, P. E., Potempa, J., Travis, J., Mackie, E. J. & Pike, R. N., 1998, In : FEBS Letters. p. 45 - 48 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Cleavage and activation of proteinase-activated receptor-2 on human neutrophils by gingipain-R from Porphyromonas gingivalis

Lourbakos, A., Chinni, C., Thompson, P., Potempa, J., Travis, J., MacKie, E. J. & Pike, R. N., 11 Sep 1998, In : FEBS Letters. 435, 1, p. 45-48 4 p.

Research output: Contribution to journalArticleResearchpeer-review

136 Citations (Scopus)

The N-terminal segment of antithrombin acts as a steric gate for the bining of heparin

Fitton, H. L., Skinner, R., Dafforn, T. R., Jin, L. & Pike, R. N., 1 Jan 1998, In : Protein Science. 7, 3, p. 782-788 7 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)
1999

Binding affinity of gingipain proteinase-adhesins to a range of proteins

Ally, N. & Pike, R. N., 1999, p. A4 - A4. 1 p.

Research output: Contribution to conferenceAbstract

Cartilage proteoglycan degradation by a mouse transformed macrophage cell line is mediated by macrophage metalloelastase

Janusz, M. J., Hare, M., Durham, S. L., Potempa, J., McGraw, W., Pike, R., Travis, J. & Shapiro, S. D., 14 Jun 1999, In : Inflammation Research. 48, 5, p. 280-288 9 p.

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Cysteine proteinase inhibitors kill cultured bloodstream forms of "Trypanosoma brucei brucei"

Troeberg, L., Morty, R. E., Pike, R. N., Lonsdale-Eccles, J. D., Palmer, J. T., McKerrow, J. H. & Coetzer, T. H., 1999, In : Experimental Parasitology. p. 349 - 355 7 p.

Research output: Contribution to journalArticleResearchpeer-review

55 Citations (Scopus)

Engineering a novel factor Xa inhibitor

Smith, S. R., Whisstock, J. C., Lawrenson, I. D., Hopkins, P., Ludeman, J., Bottomley, S. P. & Pike, R. N., 1999, p. B12 - B12. 1 p.

Research output: Contribution to conferenceAbstract

Serpins in the "Caenorhabditis elegans" genome

Whisstock, J. C., Irving, J., Bottomley, S. P., Pike, R. N. & Lesk, A. M., 1999, In : Proteins: Structure, Function, and Bioinformatics. p. 31 - 41 11 p.

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

The importance of strand 1C in the inhibitory mechanism of alpha1-antitrypsin

Lawrenson, I. D., Whisstock, J. C., Pike, R. N. & Bottomley, S. P., 1999, p. C38 - C38. 1 p.

Research output: Contribution to conferenceAbstract

The mechanism of anticoagulant activation of antithrombin by heparin

Whisstock, J. C., Lesk, A. M. & Pike, R. N., 1999, p. C39 - C39. 1 p.

Research output: Contribution to conferenceAbstract

The phylogeny of the serpin superfamily

Irving, J., Boyd, S., Pike, R. N. & Whisstock, J. C., 1999, p. C40 - C40. 1 p.

Research output: Contribution to conferenceAbstract

2000

A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica

Smooker, P. M., Whisstock, J. C., Irving, J., Siyaguna, S., Spithill, T. W. & Pike, R. N., 2000, In : Protein Science. p. 1 - 6 6 p.

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)