1989 …2019

Research output per year

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Research Output

2019

Protease-associated import systems are widespread in Gram-negative bacteria

Grinter, R., Leung, B., Wijeyewickrema, L. C., Littler, D., Beckham, S., Pike, R. N., Walker, D., Greening, C. & Lithgow, T., 1 Oct 2019, In : PLoS Genetics. 15, 10, 24 p., e1008435.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
1 Citation (Scopus)

Twenty years of bioinformatics research for protease-specific substrate and cleavage site prediction: a comprehensive revisit and benchmarking of existing methods

Li, F., Wang, Y., Li, C., Marquez-Lago, T. T., Leier, A., Rawlings, N. D., Haffari, G., Revote, J., Akutsu, T., Chou, K-C., Purcell, A. W., Pike, R. N., Webb, G. I., Smith, A. I., Lithgow, T., Daly, R. J., Whisstock, J. C. & Song, J., 27 Nov 2019, In : Briefings in Bioinformatics. 20, 6, p. 2150-2166 17 p.

Research output: Contribution to journalArticleResearchpeer-review

2018

PROSPERous: high-throughput prediction of substrate cleavage sites for 90 proteases with improved accuracy

Song, J., Li, F., Leier, A., Marquez-Lago, T., Akutsu, T., Haffari, G., Chou, K-C., Webb, G. I. B. & Pike, R. N., 15 Feb 2018, In : Bioinformatics. 34, 4, p. 684-687 4 p.

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)
2017

Recruitment of human C1 esterase inhibitor controls complement activation on blood stage plasmodium falciparum merozoites

Kennedy, A. T., Wijeyewickrema, L. C., Huglo, A., Lin, C., Pike, R., Cowman, A. F. & Tham, W-H., 15 Jun 2017, In : Journal of Immunology. 198, 12, p. 4728-4737 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
9 Citations (Scopus)

The structural basis for complement inhibition by gigastasin, a protease inhibitor from the giant Amazon leech

Pang, S. S., Wijeyewickrema, L. C., Hor, L., Tan, S., Lameignere, E., Conway, E. M., Blom, A. M., Mohlin, F. C., Liu, X., Payne, R. J., Whisstock, J. C. & Pike, R. N., 1 Dec 2017, In : Journal of Immunology. 199, 11, p. 3883-3891 9 p.

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)
2016

Polyphosphate is a novel cofactor for regulation of complement by a serpin, C1 inhibitor

Wijeyewickrema, L. C., Lameignere, E., Hor, L., Duncan, R. C., Shiba, T., Travers, R. J., Kapopara, P. R., Lei, V., Smith, S. A., Kim, H., Morrissey, J. H., Pike, R. N. & Conway, E. M., 29 Sep 2016, In : Blood. 128, 13, p. 1766-1776 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
24 Citations (Scopus)

Protein unfolding is essential for cleavage within the α-helix of a model protein substrate by the serine protease, thrombin

Robertson, A. L., Headey, S. J., Ng, N. M., Wijeyewickrema, L. C., Scanlon, M. J., Pike, R. N. & Bottomley, S. P., Mar 2016, In : Biochimie. 122, p. 227-234 8 p.

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase

Modak, J. K., Rut, W., Wijeyewickrema, L. C., Pike, R. N., Drag, M. & Roujeinikova, A., Feb 2016, In : Biochimie. 121, p. 60-71 12 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

TACTIC: a multicentre, open-label, single-arm phase II trial of panitumumab, cisplatin, and gemcitabine in biliary tract cancer

Ferraro, D., Goldstein, D., O’Connell, R. L., Zalcberg, J. R., Sjoquist, K. M., Tebbutt, N. C., Grimison, P., McLachlan, S., Lipton, L. L., Vasey, P., Gebski, V. J., Aiken, C., Cronk, M., Ng, S., Karapetis, C. S., Shannon, J. & behalf of the Australasian Gastro-Intestinal Trials Group, 1 Aug 2016, In : Cancer Chemotherapy and Pharmacology. 78, 2, p. 361-367 7 p.

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)
2015

Investigation of the mechanism of interaction between Mannose-binding lectin-associated serine protease-2 and complement C4

Drentin, N. C., Conroy, P., Gunzburg, M. J., Pike, R. N. & Wijeyewickrema, L. C., 2015, In : Molecular Immunology. 67, 2, p. 287 - 293 7 p.

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

The protease cathepsin L regulates Th17 cell differentiation

Hou, L., Cooley, J., Swanson, R., Ong, P. C., Pike, R. N., Bogyo, M., Olson, S. T. & Remold-O'Donnell, E., 2015, In : Journal of Autoimmunity. 65, p. 56 - 63 8 p.

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)
2014

Scabies mite inactive serine proteases are potent inhibitors of the human complement lectin pathway

Reynolds, S., Pike, R. N., Mika, A., Blom, A., Hofmann, A., Wijeyewickrema, L. C., Kemp, D. J. & Fischer, K., 2014, In : PLoS Neglected Tropical Diseases. 8, 5, 8 p., e2872.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
22 Citations (Scopus)

The role of the lys628 (192) residue of the complement protease, c1s, in interacting with Peptide and protein substrates

Wijeyewickrema, L. C., Duncan, R. C. & Pike, R. N., 2014, In : Frontiers in Immunology. 5, 444, p. 1 - 6 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
1 Citation (Scopus)

Total synthesis of homogeneous variants of hirudin P6: a post-translationally modified anti-thrombotic leech-derived protein

Hsieh, Y. SY., Wijeyewickrema, L. C., Wilkinson, B., Pike, R. N. & Payne, R. J., 2014, In : Angewandte Chemie - International Edition. 53, 15, p. 3947 - 3951 5 p.

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)
2013

A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis

Scally, S., Petersen, J., Law, S. C. CHENG., Dudek, N. L., Nel, H. J., Loh, K. L., Wijeyewickrema, L. C., Eckle, S. B. G., van Heemst, J., Pike, R. N., McCluskey, J., Toes, R. E., La Gruta, N. L., Purcell, A. W., Reid, H. H., Thomas, R. & Rossjohn, J., 2013, In : Journal of Experimental Medicine. 210, 12, p. 2569 - 2582 14 p.

Research output: Contribution to journalArticleResearchpeer-review

210 Citations (Scopus)

A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4

Perry, A. J., Wijeyewickrema, L. C., Wilmann, P. G., Gunzburg, M. J., D'Andrea, L. P., Irving, J., Pang, S. S., Duncan, R. C., Wilce, J. A., Whisstock, J. & Pike, R. N., 2013, In : The Journal of Biological Chemistry. 288, 22, p. 15821 - 15829 9 p.

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS

Dunstan, R. A., Heinz, E., Wijeyewickrema, L. C., Pike, R. N., Purcell, A. W., Evans, T. J., Praszkier, J., Robins-Browne, R. M., Strugnell, R. A., Korotkov, K. V. & Lithgow, T. J., 2013, In : PLoS Pathogens. 9, 1 (Art # e1003117), p. 1 - 14 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
43 Citations (Scopus)

Gingipain K

Pike, R. N. & Potempa, J., 2013, Handbook of Proteolytic Enzymes. Rawlings, N. D. & Salvesen, G. (eds.). 3rd ed. UK: Academic Press, Vol. 2. p. 2337 - 2344 8 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

4 Citations (Scopus)

Molecular determinants of the substrate specificity of the complement-initiating protease, C1r

Wijeyewickrema, L. C., Yongqing, T., Tran, P. T., Thompson, P., Viljoen, J. E., Coetzer, T. H., Duncan, R. C., Kass, I., Buckle, A. M. & Pike, R. N., 2013, In : The Journal of Biological Chemistry. 288, 22, p. 15571 - 15580 10 p.

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates

Langendorf, C. G., Tuck, K. L., Key, T., Fenalti, G., Pike, R. N., Rosado, C. J., Wong, A. SM., Buckle, A. M., Law, R. H. P. & Whisstock, J., 2013, In : Bioscience Reports. 33, 1 (Art # A13), p. 137 - 149 13 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

The molecular switches controlling the interaction between complement proteases of the classical and lectin pathways and their substrates

Pike, R. N. & Wijeyewickrema, L. C., 2013, In : Current Opinion in Structural Biology. 23, 6, p. 820 - 827 8 p.

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

The x-ray crystal structure of mannose-binding lectin-associated serine proteinase-3 reveals the structural basis for enzyme inactivity associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) syndrome

Yongqing, T., Wilmann, P. G., Reeve, S. B., Coetzer, T. H., Smith, A. I., Whisstock, J., Pike, R. N. & Wijeyewickrema, L. C., 2013, In : The Journal of Biological Chemistry. 288, 31, p. 22399 - 22407 9 p.

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Thrombin inhibits osteoclast differentiation through a non-proteolytic mechanism

Sivagurunathan, S., Pagel, C. N., Loh, L. H., Wijeyewickrema, L. C., Pike, R. N. & Mackie, E. J., 2013, In : Journal of Molecular Endocrinology. 50, 3, p. 347 - 359 13 p.

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)
2012

Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1-P4 specificity reveals an unusual preference

Norbury, L. J., Hung, A., Beckham, S. A., Pike, R. N., Spithill, T. W., Craik, C. S., Choe, Y., Fecondo, J. V. & Smooker, P. M., 2012, In : Biochimie. 94, 5, p. 1119 - 1127 9 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Hsieh, Y. SY., Taleski, D., Wilkinson, B., Wijeyewickrema, L. C., Adams, T. E., Pike, R. N. & Payne, R. J., 2012, In : Chemical Communications. 48, 10, p. 1547 - 1549 3 p.

Research output: Contribution to journalArticleOtherpeer-review

15 Citations (Scopus)

Identification of a catalytic exosite for complement component c4 on the serine protease domain of c1s

Duncan, R. C., Mohlin, F. C., Taleski, D., Coetzer, T. H., Huntington, J. A., Payne, R. J., Blom, A. M., Pike, R. N. & Wijeyewickrema, L. C., 2012, In : Journal of Immunology. 189, 5, p. 2365 - 2373 9 p.

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Mannose-binding lectin serine proteases and associated proteins of the lectin pathway of complement: two genes, five proteins and many functions?

Yongqing, T., Drentin, N., Duncan, R. C., Wijeyewickrema, L. C. & Pike, R. N., 2012, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1824, 1, p. 253 - 262 10 p.

Research output: Contribution to journalArticleResearchpeer-review

50 Citations (Scopus)

Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4

Duncan, R. C., Bergstrom, F. C., Coetzer, T. H., Blom, A. M., Wijeyewickrema, L. C. & Pike, R. N., 2012, In : Molecular Immunology. 49, 4, p. 593 - 600 8 p.

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Novel scabies mite serpins inhibit the three pathways of the human complement system

Mika, A., Reynolds, S. L., Mohlin, F. C., Willis, C., Swe, P. M., Pickering, D. A., Halilovic, V., Wijeyewickrema, L. C., Pike, R. N., Blom, A. M., Kemp, D. J. & Fischer, K., 2012, In : PLoS ONE. 7, 7, 15 p., e40489.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
40 Citations (Scopus)

PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites

Song, J., Tan, H., Perry, A. J., Akutsu, T., Webb, G. I., Whisstock, J. C. & Pike, R. N., 2012, In : PLoS ONE. 7, 11, 23 p., e50300.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
131 Citations (Scopus)

Proteinase-activated receptor-2 is required for normal osteoblast and osteoclast differentiation during skeletal growth and repair

Georgy, S. R., Pagel, C., Ghasem-Zadeh, A., Zebaze, R., Pike, R., Sims, N. & Mackie, E., 2012, In : Bone. 50, 3, p. 704 - 712 9 p.

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

The x-ray crystal structure of full-length human plasminogen

Law, R. HP., Caradoc-Davies, T. T., Cowieson, N. P., Horvath, A. J., Quek, A. J., Amarante Da Encarnacao, J., Steer, D. L., Cowan, A. D., Zhang, Q., Lu, B. GC., Pike, R. N., Smith, A. I., Coughlin, P. B. & Whisstock, J. C., 2012, In : Cell Reports. 1, 3, p. 185 - 190 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
111 Citations (Scopus)
2011

Adult and juvenile Fasciola cathepsin L proteases: Different enzymes for different roles

Norbury, L. J., Beckham, S. A., Pike, R. N., Grams, R., Spithill, T. W., Fecondo, J. V. & Smooker, P. M., 2011, In : Biochimie. 93, 3, p. 604 - 611 8 p.

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Bioinformatic approaches for predicting substrates of proteases

Song, J., Tan, H., Boyd, S. E., Shen, H., Mahmood, K., Webb, G., Akutsu, T., Whisstock, J. & Pike, R., 2011, In : Journal of Bioinformatics and Computational Biology. 9, 1, p. 149 - 178 30 p.

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate

Ng, N., Pierce, M. J., Webb, G., Ratnikov, B., Wijeyewickrema, L. C., Duncan, R., Robertson, A. L., Bottomley, S. P., Boyd, S. E. & Pike, R. N., 2011, In : Biochemistry. 50, 48, p. 10499 - 10507 9 p.

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Methods to measure the kinetics of protease inhibition by serpins

Horvath, A. J., Lu, B. GC., Pike, R. N. & Bottomley, S. P., 2011, Methods in Enzymology, Volume 501: Serpin Structure and Evolution. Whisstock, J. C. & Bird, P. I. (eds.). USA: Academic Press, p. 223 - 235 13 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

10 Citations (Scopus)

Predicting serpin/protease interactions

Song, J., Matthews, A., Reboul, C. F., Kaiserman, D., Pike, R. N., Bird, P. I. & Whisstock, J. C., 2011, Methods in Enzymology, Volume 501: Serpin Structure and Evolution. Whisstock, J. C. & Bird, P. I. (eds.). USA: Academic Press, p. 237 - 273 37 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

6 Citations (Scopus)

S1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction

Wong, W., Wijeyewickrema, L. C., Kennan, R. M., Reeve, S. B., Steer, D. L., Reboul, C., Smith, A. I., Pike, R. N., Rood, J. I., Whisstock, J. C. & Porter, C. J., 2011, In : The Journal of Biological Chemistry. 286, 49, p. 42180 - 42187 8 p.

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Serpins and the complement system

Beinrohr, L., Murray-Rust, T., Dyksterhuis, L., Zavodszky, P., Gal, P., Pike, R. & Wijeyewickrema, L., 2011, Methods in Enzymology, Volume 499: Biology of Serpins. Whisstock, J. C. & Bird, P. I. (eds.). 1st ed. UK: Academic Press, p. 55 - 75 21 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

8 Citations (Scopus)

The lysine-specific gingipain of porphyromonas gingivalis : Importance to pathogenicity and potential strategies for inhibition

Yongqing, T., Potempa, J., Pike, R. & Wijeyewickrema, L., 2011, Cysteine Proteases of Pathogenic Organisms. Robinson, M. W. & Dalton, J. P. (eds.). 1st ed. USA: Springer, p. 15 - 29 15 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

22 Citations (Scopus)
2010

Cathepsin B proteases of flukes: The key to facilitating parasite control?

Smooker, P. M., Jayaraj, R., Pike, R. N. & Spithill, T. W., 2010, In : Trends in Parasitology. 26, 10, p. 506 - 514 9 p.

Research output: Contribution to journalArticleResearchpeer-review

51 Citations (Scopus)

Fluorinated beta2- and beta3-amino acids: Synthesis and inhibition of alpha-chymotrypsin

Peddie, V., Pietsch, M., Bromfield, K. M., Pike, R. N., Duggan, P. J. & Abell, A. D., 2010, In : Synthesis. 2010, 11, p. 1845 - 1859 15 p.

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Highlight: The Biology of Proteolytic Systems

Pike, R. N. & Whisstock, J., 2010, In : Biological Chemistry. 391, 8, p. 837 - 837 1 p.

Research output: Contribution to journalLetterOther

Protease-activated receptor 2 has pivotal roles in cellular mechanisms involved in experimental periodontitis

Wong, D. M., Tam, V., Lam, R., Walsh, K. A., Tatarczuch, L., Pagel, C. N., Reynolds, E. C., O'Brien-Simpson, N. M., Mackie, E. J. & Pike, R. N., 2010, In : Infection and Immunity. 78, p. 629 - 638 9 p.

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Proteinase-activated receptor-2 (PAR2) and mouse osteoblasts: Regulation of cell function and lack of specificity of PAR2- activating peptides

Georgy, S. R., Pagel, C. N., Wong, D. M., Sivagurunathan, S., Loh, L. H., Myers, D. E., Hollenberg, M. D., Pike, R. N. & Mackie, E. J., 2010, In : Clinical and Experimental Pharmacology and Physiology. 37, 3, p. 328 - 336 9 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Synthesis of "difficult" fluorescence quenched substrates of granzyme C

Northfield, S., Roberts, K., Mountford, S., Hughes, R., Kaiserman, D., Mangan, M., Pike, R., Bird, P. & Thompson, P., 2010, In : International Journal of Peptide Research and Therapeutics. 16, p. 159 - 165 7 p.

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates

Kennan, R. M., Wong, W., Dhungyel, O., Han, X., Wong, D. M., Parker, D., Rosado, C. J., Law, R. HP., McGowan, S., Reeve, S. B., Levina, V., Powers, G. A., Pike, R. N., Bottomley, S. P., Smith, A. I., Marsh, I., Whittington, R. J., Whisstock, J., Porter, C. J. & Rood, J. I., 2010, In : PLoS Pathogens. 6, 11, p. 1 - 12 12 p.

Research output: Contribution to journalArticleResearchpeer-review

55 Citations (Scopus)

Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni

Tchoubrieva, E. B., Ong, P. C., Pike, R. N., Brindley, P. J. & Kalinna, B. H., 2010, In : Cellular and Molecular Life Sciences. 67, 21, p. 3739 - 3748 10 p.

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)
2009

A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites

Beckham, S. A., Piedrafita, D. M., Phillips, C. I., Samarawickrema, N., Law, R. H. P., Smooker, P. M., Quinsey, N. S., Irving, J. A., Greenwood, D. L., Verhelst, S. H. L., Bogyo, M., Turk, B., Coetzer, T. H., Wijeyewickrema, L. C., Spithill, T. W. & Pike, R. N., 2009, In : International Journal of Biochemistry and Cell Biology. 41, 7, p. 1601 - 1612 12 p.

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)