Projects per year
Fluorescent protein technology has revolutionised the way in which we carry out experiments in the life sciences, and few areas of biological research remain untouched by the technology. Fluorescent proteins such as the green fluorescent protein (GFP) cloned from the jellyfish Aequorea victoria, have been engineered to produce proteins with different fluorescent properties (for example see picture) useful for sensing a vast range of events in living cells. GFP is just one member of the protein superfamily found in marine organisms. Although each member folds to form the same 11-stranded b-barrel a variety of different chromophores (the light emitting component buried inside the barrel) together with the complex network of interactions between the chromophore and the surrounding amino acid side-chains (the protein matrix) determine the myriad range of optical properties.
Our aim is twofold: (a) understand the complex and subtle relationship between FP structure and optical properties, and (b) use newly acquired knowledge to design and engineer new FPs for novel biotechnology applications. In particular we are exploring their use in the fields of autophagy research, super-resolution microscopy and optogenetics. In the new and exciting field of optogenetics light-sensitive probes are used together with focussed light to switch processes 'on' and 'off' in living cells, tissues and intact organisms.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Research area keywords
- fluorescent protein
Travel from 18 to 27 September 2000
16/10/00 → …
What are the roles of laboratory classes in biomedical science education?
Macaulay, J., Gibbons, J., Davis, E., Johanesen, P., Murphy, K., Choate, J., Prescott, M. & Abud, H.
Monash University – Internal Faculty Contribution
1/04/18 → 31/03/19
How Burkholderia pseudomallei subverts host ubiquitination and autophagy pathways
Devenish, R., Boyce, J., Kleifeld, O. & Prescott, M.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/13 → 31/12/16
Disruption of the Burkholderia pseudomallei two-component signal transduction system BbeR-BbeS leads to increased extracellular DNA secretion and altered biofilm formationAlwis, P. A., Treerat, P., Gong, L., Deveson Lucas, D., Allwood, E. M., Prescott, M., Devenish, R. J., Adler, B. & Boyce, J. D., Mar 2020, In: Veterinary Microbiology. 242, 11 p., 108603.
Research output: Contribution to journal › Article › Research › peer-review
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., Adhihetty, P. J., Adler, S. G., Agam, G., Agarwal, R., Aghi, M. K., Agnello, M., Agostinis, P., Aguilar, P. V., Aguirre-Ghiso, J. A., Airoldi, E. M., & 30 others, 2016, In: Autophagy. 12, 1, p. 1-222 222 p.
Research output: Contribution to journal › Review Article › Research › peer-reviewOpen Access
Perturbation of the two-component signal transduction system, BprRS, results in attenuated virulence and motility defects in Burkholderia pseudomalleiLazar Adler, N. R., Allwood, E. M., Deveson Lucas, D., Harrison, P., Watts, S., Dimitropoulos, A., Treerat, P., Alwis, P., Devenish, R. J., Prescott, M., Govan, B., Adler, B., Harper, M. & Boyce, J. D., 4 May 2016, In: BMC Genomics. 17, 17 p., 331.
Research output: Contribution to journal › Article › Research › peer-reviewOpen AccessFile
Autophagy: starvation relieves transcriptional repression of ATG genesDevenish, R. J. & Prescott, M., 2015, In: Current Biology. 25, 6, p. 238 - 240 3 p.
Research output: Contribution to journal › Article › Other › peer-reviewOpen Access
Burkholderia pseudomallei type III secretion system cluster 3 ATPase BsaS, a chemotherapeutic target for small-molecule ATPase inhibitorsGong, L., Lai, S., Treerat, P., Prescott, M., Adler, B., Boyce, J. D. & Devenish, R. J., 2015, In: Infection and Immunity. 83, 4, p. 1276 - 1285 10 p.
Research output: Contribution to journal › Article › Research › peer-reviewOpen Access