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Research Output 1997 2019

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2005
31 Citations (Scopus)

The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition

Fulton, K. F., Buckle, A. M., Cabrita, L. D., Irving, J. A., Butcher, R. E., Smith, A. I., Reeve, S. B., Lesk, A. M., Bottomley, S. P., Rossjohn, J. & Whisstock, J. C., 2005, In : Journal of Biological Chemistry. 280, 9, p. 8435 - 8442 8 p.

Research output: Contribution to journalArticleResearchpeer-review

55 Citations (Scopus)

The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix

Law, R. H. P., Irving, J. A., Buckle, A. M., Ruzyla, K., Buzza, M. S., Bashtannyk-Puhalovich, T. A., Beddoe, T. C., Nguyen, K-N., Worrall, M. D., Bottomley, S. P., Bird, P. I., Rossjohn, J. & Whisstock, J. C., 2005, In : Journal of Biological Chemistry. 280, 23, p. 22356 - 22364 9 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

The matrix refolded

Buckle, A. M., Devlin, G. L., Jodun, R. A., Fulton, K. F., Faux, N. G., Whisstock, J. C. & Bottomley, S. P., 2005, In : Nature Methods. 2, 1, p. 3 - 3 1 p.

Research output: Contribution to journalArticleResearchpeer-review

67 Citations (Scopus)

The murine orthologue of human antichymotrypsin: a structural paradigm for CLADE A3 serpins

Horvath, A. J., Irving, J. A., Rossjohn, J., Law, R. H. P., Bottomley, S. P., Quinsey, N. S., Pike, R. N., Coughlin, P. B. & Whisstock, J., 2005, In : Journal of Biological Chemistry. 280, 52, p. 43168 - 43178 11 p.

Research output: Contribution to journalArticleResearchpeer-review

84 Citations (Scopus)

The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane

Ivetac, I., Munday, A. D., Kisseleva, M. V., Zhang, X-M., Luff, S. E., Tiganis, T., Whisstock, J. C., Rowe, T., Majerus, P. W. & Mitchell, C. A., 2005, In : Molecular Biology of the Cell. 16, 5, p. 2218 - 2233 16 p.

Research output: Contribution to journalArticleResearchpeer-review

2004
87 Citations (Scopus)

Antithrombin: in control of coagulation

Quinsey, N. S., Greedy, A. L., Bottomley, S. P., Whisstock, J. C. & Pike, R. N., 2004, In : International Journal of Biochemistry and Cell Biology. 36, 3, p. 386 - 389 4 p.

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

CLIMS: Crystallography Laboratory Information Management System

Fulton, K. F., Ervine, S., Faux, N. G., Forster, R., Ly, W., Robilliard, L., Sonsini, J., Whelan, D., Whisstock, J. C. & Buckle, A. M., 2004, In : Acta Crystallographica Section D: Biological Crystallography. 60, 9, p. 1691 - 1693 3 p.

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Computational analysis of evolution and conservation in a protein superfamily

Irving, J. A., Askew, D. J. & Whisstock, J. C., 2004, In : Methods. 32, 2, p. 73 - 92 20 p.

Research output: Contribution to journalArticleResearchpeer-review

135 Citations (Scopus)

Human clade B serpins (ov-serpins) belong to a cohort of evolutionarily dispersed intracellular proteinase inhibitor clades that protect cells from promiscuous proteolysis

Silverman, G. A., Whisstock, J. C., Askew, D. J., Pak, S. C., Luke, C. J., Cataltepe, S., Irving, J. A. & Bird, P. I., 2004, In : Cellular and Molecular Life Sciences. 61, 3, p. 301 - 325 25 p.

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Progressive multiple alignment using sequence triplet optimizations and three-residue exchange costs

Konagurthu, A., Whisstock, J. & Stuckey, P., 2004, In : Journal of Bioinformatics and Computational Biology. 2, 4, p. 719 - 745 27 p.

Research output: Contribution to journalArticleResearchpeer-review

51 Citations (Scopus)

Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3

Chow, M. K. M., Mackay, J. P., Whisstock, J. C., Scanlon, M. J. & Bottomley, S. P., 2004, In : Biochemical and Biophysical Research Communications. 322, 3, p. 387 - 394 8 p.

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

The human serpin proteinase inhibitor-9 self-associates at physiological temperatures

Benning, L. N., Whisstock, J. C., Sun, J., Bird, P. I. & Bottomley, S. P., 2004, In : Protein Science. 13, 7, p. 1859 - 1864 6 p.

Research output: Contribution to journalArticleResearchpeer-review

2003
273 Citations (Scopus)

A structural basis for the selection of dominant T cell receptors in antiviral immunity

Kjer-Nielsen, L., Clements, C. S., Purcell, A. W., Brooks, A. G., Whisstock, J. C., Burrows, S. R., McCluskey, J. & Rossjohn, J., 2003, In : Immunity. 18, 1, p. 53 - 64 12 p.

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes

Ally, N., Whisstock, J. C., Sieprawskalupa, M., Potempa, J., Le Bonniec, B. F., Travis, J. & Pike, R. N., 2003, In : Biochemistry. 42, 40, p. 11693 - 11700 8 p.

Research output: Contribution to journalArticleResearchpeer-review

68 Citations (Scopus)

Cloning and expression of the major secreted cathepsin B-Iike protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection

Law, R. H. P., Smooker, P. M., Irving, J. A., Piedrafita, D., Ponting, R., Kennedy, N. J., Whisstock, J. C., Pike, R. N. & Spithill, T. W., 2003, In : Infection and Immunity. 71, 12, p. 6921 - 6932 12 p.

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Determination of the P'1, P'2 and P'3 subsite-specificity of factor Xa

Ludeman, J. P., Pike, R. N., Bromfield, K. M., Duggan, P. J., Cianci, J., Le Bonniec, B., Whisstock, J. C. & Bottomley, S. P., 2003, In : International Journal of Biochemistry and Cell Biology. 35, 2, p. 221 - 225 5 p.

Research output: Contribution to journalArticleResearchpeer-review

67 Citations (Scopus)

Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis

Welss, T., Sun, J., Irving, J. A., Blum, R., Smith, A. I., Whisstock, J. C., Pike, R. N., Von Mikecz, A., Ruzicka, T., Bird, P. I. & Abts, H. F., 2003, In : Biochemistry. 42, 24, p. 7381 - 7389 9 p.

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates

O'Brien, G., Quinsey, N. S., Whisstock, J. C. & Pike, R. N., 2003, In : Biochemistry. 42, 50, p. 14939 - 14945 7 p.

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Introduction of a mutation in the shutter region of antithrombin (Phe77 Leu) increases affinity for heparin and decreases thermal stability

Quinsey, N. S., Fitton, H. L., Coughlin, P. B., Whisstock, J. C., Dafforn, T. R., Carrell, R. W., Bottomley, S. P. & Pike, R. N., 2003, In : Biochemistry. 42, 34, p. 10169 - 10173 5 p.

Research output: Contribution to journalArticleResearchpeer-review

142 Citations (Scopus)

Molecules in focus: glycoprotein Ib-IX-V

Andrews, R. K., Gardiner, E. E., Shen, Y., Whisstock, J. C. & Berndt, M. C., 2003, In : International Journal of Biochemistry and Cell Biology. 35, 8, p. 1170 - 1174 5 p.

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Role of the M-loop and reactive center loop domains in the folding and bridging of nucleosome arrays by MENT

Springhetti, E. M., Istomina, N. E., Whisstock, J. C., Nikitina, T., Woodcock, C. L. & Grigoryev, S. A., 2003, In : Journal of Biological Chemistry. 278, 44, p. 43384 - 43393 10 p.

Research output: Contribution to journalArticleResearchpeer-review

43 Citations (Scopus)

The 1.5 crystal structure of a prokaryote serpin: controlling conformational change in a heated environment

Irving, J. A., Cabrita, L. D., Rossjohn, J., Pike, R. N., Bottomley, S. P. & Whisstock, J. C., 2003, In : Structure. 11, 4, p. 387 - 397 11 p.

Research output: Contribution to journalArticleResearchpeer-review

52 Citations (Scopus)

The evolution of enzyme specificity in Fasciola spp.

Irving, J. A., Spithill, T. W., Pike, R. N., Whisstock, J. C. & Smooker, P. M., 2003, In : Journal of Molecular Evolution. 57, 1, p. 1 - 15 15 p.

Research output: Contribution to journalArticleResearchpeer-review

2002
51 Citations (Scopus)

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: Engineering α1-antitrypsin to inhibit cathepsin proteases

Irving, J. A., Pike, R. N., Dai, W., Brömme, D., Worrall, D. M., Silverman, G. A., Coetzer, T. H. T., Dennison, C., Bottomley, S. P. & Whisstock, J. C., 16 Apr 2002, In : Biochemistry. 41, 15, p. 4998-5004 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Evidence that serpin architecture intrinsically supports papain-like cystein protease inhibition: Engineering 1- abutrypsin to inhibit acathepsin proteases

Irving, J., Pike, R. N., Dai, W., Bromme, D., Worrall, M. D., Silverman, G. A., Coetzer, T. H., Dennison, C., Bottomley, S. P. & Whisstock, J. C., 2002, In : Biochemistry. 41, 15, p. 4998 - 5004 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Inhibitory activity of a heterchromatin-associated serpin (MENT) against papain like cystein proteinases affects chromatin structure and blocks cell proliferation

Irving, J. A., Shushanov, S., Pike, R. N., Popova, E. Y., Bromme, D., Coetzer, T. H., Bottomley, S. P., Boulynko, I. A., Grigoryev, S. A. & Whisstock, J. C., 2002, In : Journal of Biological Chemistry. 277, 15, p. 13192 - 13201 10 p.

Research output: Contribution to journalArticleResearchpeer-review

79 Citations (Scopus)

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

Irving, J. A., Shushanov, S. S., Pike, R. N., Popova, E. Y., Brömme, D., Coetzer, T. H. T., Bottomley, S. P., Boulynko, I. A., Grigoryev, S. A. & Whisstock, J. C., 12 Apr 2002, In : Journal of Biological Chemistry. 277, 15, p. 13192-13201 10 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Molecular determinants of the mechanisms underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

Quinsey, N. S., Whisstock, J. C., Le Bonniec, B., Louvain, V., Bottomley, S. P. & Pike, R. N., 2002, In : Journal of Biological Chemistry. 277, 18, p. 15971 - 15978 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Molecular Modeling of the Seven Tandem Leucine-Rich Repeats within the Ligand-Binding Region of Platelet Glycoprotein Ib

Whisstock, J. C., Shen, Y., Lopez, J. A., Andrews, R. K. & Berndt, M. C., 2002, In : Thrombosis and Haemostasis. 87, 2, p. 329 - 333 5 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Molecular modeling of the seven tandem leucine-rich repeats within the ligand-binding region of platelet glycoprotein Ibα

Whisstock, J. C., Shen, Y., López, J. A., Andrews, R. K. & Berndt, M. C., 21 Feb 2002, In : Thrombosis and Haemostasis. 87, 2, p. 329-333 5 p.

Research output: Contribution to journalArticleResearchpeer-review

40 Citations (Scopus)

Probing the role of the F-Helix in serpin stability through a single tryptophan substitution

Cabrita, L., Whisstock, J. C. & Bottomley, S. P., 2002, In : Biochemistry. 41, 14, p. 4575 - 4581 7 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Serpins: finely balanced conformational traps

Pike, R. N., Bottomley, S. P., Irving, J., Bird, P. I. & Whisstock, J. C., 2002, In : IUBMB Life. 54, 1, p. 1 - 7 7 p.

Research output: Contribution to journalArticleResearchpeer-review

93 Citations (Scopus)

Serpins in Prokaryotes

Irving, J., Steenbakkers, P. J., Lesk, A. M., Op Den Camp, H. J., Pike, R. N. & Whisstock, J. C., 2002, In : Molecular Biology and Evolution. 19, 11, p. 1881 - 1890 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Subunit fusion are functionally incorporated into mitochondrial F1F0 synthase arguing against a rigid cap structure at the top of F1

Prescott, M., Nowakowski, S., Gavin, P., Nagley, P., Whisstock, J. C. & Devenish, R. J., 2002, In : Journal of Biological Chemistry. 278, 1, p. 251 - 256 6 p.

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionizatin Mass Spectronometry Techniques Based on Isotope Exchange Effects

Hearn, M. T. W., Quirino, J. P., Whisstock, J. C. & Terabe, S., 2002, In : Analytical Chemistry. 74, 6, p. 1467 - 1475 9 p.

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)

The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases

Al-Khunaizi, M., Luke, C. J., Askew, Y. S., Pak, S. C., Cataltepe, S., Miller, D. R., Mills, D. R., Tsu, C., Bromme, D., Irving, J., Whisstock, J. C. & Silverman, G. A., 2002, In : Biochemistry. 41, 9, p. 3189 - 3199 11 p.

Research output: Contribution to journalArticleResearchpeer-review

The structure and function of catalytic domains within inositol polyphosphate 5- phosphates

Whisstock, J. C., Wiradjaja, F., Waters, J. & Gurung, R., 2002, In : IUBMB Life. 53, 1, p. 15 - 23 9 p.

Research output: Contribution to journalArticleResearchpeer-review

2001

Characterization and functional analysis of Serp3: a novel myxoma virus-encoded serpin invloved in virulence

Guerin, J-L., Gelfi, J., Camus, C., Delverdier, M., Whisstock, J. C., Amardeihl, M-F., Py, R., Bertagnole, S. & Messud-Petit, F., 2001, In : Journal of General Virology. 82, p. 1407-1417 11 p.

Research output: Contribution to journalArticleResearchpeer-review

41 Citations (Scopus)

Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: Identification of a myotubularin-related protein lacking catalytic activity

Nandurkar, H., Caldwell, K. K., Whisstock, J. C., Layton, M. J., Gaudet, E. A., Norris, F. A., Majerus, P. W. & Mitchell, C. A., 2001, In : Proceedings of the National Academy of Sciences. 98, 17, p. 9499 - 9504 6 p.

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)

Importance of the P4' residue in human granzyme B inhibitors and substrates revealed by scanning mutagenesis of the proteinase inhibitor 9 reactive center loop

Sun, J. H., Whisstock, J. C., Harriott, P., Walker, B., Novak, A. J., Thompson, P. E., Smith, A. I. & Bird, P. I., 2001, In : Journal of Biological Chemistry. 276, 18, p. 15177 - 15184 8 p.

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Mutagenesis of the dengue virus type 2 NS3 proteinase and the production of growth-restricted virus

Matusan, A. E., Kelley, P. G., Pryor, M. J., Whisstock, J. C., Davidson, A. D. & Wright, P. J., 2001, In : Journal of General Virology. 82, p. 1647 - 1656 10 p.

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)

Protein structural alignments and functional genomics

Irving, J., Whisstock, J. C. & Lesk, A. M., 2001, In : Proteins: Structure, Function, and Bioinformatics. 42, p. 378 - 382 5 p.

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy

Ludeman, J. P., Whisstock, J. C., Hopkins, P., Le Bonniec, B. & Bottomley, S. P., 2001, In : Biophysical Journal. 80, p. 491 - 497 7 p.

Research output: Contribution to journalArticleResearchpeer-review

The role of strand 1 of the C b-sheet in the structure and function of a1- antitrypsin

Bottomley, S. P., Lawrenson, I. D., Tew, D. J., Dai, W., Whisstock, J. C. & Pike, R. N., 2001, In : Protein Science. 10, p. 2518 - 2524 7 p.

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

The role of strand 1 of the C β-sheet in the structure and function of α1-antitrypsin

Bottomley, S. P., Lawrenson, I. D., Tew, D., Dai, W., Whisstock, J. C. & Pike, R. N., 8 Dec 2001, In : Protein Science. 10, 12, p. 2518-2524 7 p.

Research output: Contribution to journalArticleResearchpeer-review

952 Citations (Scopus)

The serpins are an expanding superfamily of structurally similar but functionally diverse proteins

Silverman, G. A., Bird, P. I., Carrell, R. W., Church, F. C., Coughlin, P. B., Gettins, P. G. W., Irving, J., Lomas, D. A., Luke, C., Moyer, R. W., Pemberton, P. A., Remold-O'Donnell, E., Salvesen, G. S., Travis, J. & Whisstock, J. C., 2001, In : Journal of Biological Chemistry. 276, 36, p. 33293 - 33296 4 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail

Wiradjaja, F., Ooms, L. M., Whisstock, J. C., McColl, B. K., Helfenbaum, L., Sambrook, J., Gething, M. J. & Mitchell, C. A., 2001, In : Journal of Biological Chemistry. 276, 10, p. 7643 - 7653 11 p.

Research output: Contribution to journalArticleResearchpeer-review

2000
56 Citations (Scopus)

A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica

Smooker, P. M., Whisstock, J. C., Irving, J., Siyaguna, S., Spithill, T. W. & Pike, R. N., 2000, In : Protein Science. p. 1 - 6 6 p.

Research output: Contribution to journalArticleResearchpeer-review