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Research Output 1997 2019

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Article
1998

a1 - antitrypsin polymerisation can occur by both Loop A and C sheet mechanisms

Bottomley, S. P., Hopkins, P. & Whisstock, J. C., 1998, In : Biochemical and Biophysical Research Communications. p. 1 - 5 5 p.

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of interferon-stimulated genes

de Veer, M. J., Sim, H., Whisstock, J. C., Devenish, R. J. & Ralph, S. J., 1998, In : Genomics. p. 267 - 277 11 p.

Research output: Contribution to journalArticleResearchpeer-review

1999
5 Citations (Scopus)
51 Citations (Scopus)

Expression and purification of recombinant human granzyme B from Pichia pastoris

Sun, J., Bird, C., Buzza, M. S., McKee, K. E., Whisstock, J. C. & Bird, P. I., 1999, In : Biochemical and Biophysical Research Communications. p. 251-255 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Identification of functional regions of the thyrotropin beta-subunit recognised by two monoclonal antibodies mAb

Gomme, P. T., Thompson, P. E., Whisstock, J. C., Stanton, P. & Hearn, M. T. W., 1999, In : Journal of Peptide Research. p. 218 - 229 12 p.

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopes

Davies, J. M., Scealy, M., Cai, Y. P., Whisstock, J. C., Mackay, I. R. & Rowley, M. J., 1999, In : Molecular Immunology. p. 659 - 667 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Probing the unfolding pathway of alpha1-antitrypsin

James, E. L., Whisstock, J. C., Gore, M. G. & Bottomley, S. P., 1999, In : Journal of Biological Chemistry. p. 9482 - 9488 7 p.

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Serpins in the "Caenorhabditis elegans" genome

Whisstock, J. C., Irving, J., Bottomley, S. P., Pike, R. N. & Lesk, A. M., 1999, In : Proteins: Structure, Function, and Bioinformatics. p. 31 - 41 11 p.

Research output: Contribution to journalArticleResearchpeer-review

80 Citations (Scopus)

SH3 domains in prokaryotes

Whisstock, J. C. & Lesk, A. M., 1999, In : Trends in Biochemical Sciences. p. 132 - 133 2 p.

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)
2000
56 Citations (Scopus)

A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica

Smooker, P. M., Whisstock, J. C., Irving, J., Siyaguna, S., Spithill, T. W. & Pike, R. N., 2000, In : Protein Science. p. 1 - 6 6 p.

Research output: Contribution to journalArticleResearchpeer-review

77 Citations (Scopus)

Cleaved antitrypsin polymers at atomic resolution

Dunstone, M. A., Dai, W., Whisstock, J. C., Rossjohn, J., Pike, R. N., Feil, S. C., Le Bonniec, B. F., Parker, M. W. & Bottomley, S. P., 13 Mar 2000, In : Protein Science. 9, 2, p. 417-420 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Cleaved antitrypsin polymers at atominc resolution

Dunstone, M. A., Dai, W., Whisstock, J. C., Rossjohn, J., Pike, R. N., Feil, S. C., Le Bonniec, B., Parker, M. W. & Bottomley, S. P., 2000, In : Protein Science. p. 417 - 420 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Conformational changes in serpins: 1. The native and cleaved conformations of alpha1-antitrypsin

Whisstock, J. C., Skinner, R., Carrell, R. W. & Lesk, A. M., 2000, In : Journal of Molecular Biology. p. 685 - 699 15 p.

Research output: Contribution to journalArticleResearchpeer-review

78 Citations (Scopus)

Conformational changes in serpins: II. The mechanism of activation of antithrombin by heparin

Whisstock, J. C., Pike, R. N., Jin, L., Skinner, R., Pei, X. Y., Carrell, R. W. & Lesk, A. M., 2000, In : Journal of Molecular Biology. p. 1287 - 1305 19 p.

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)

Conformational epitopes on the diabetes autoantigen GAD65 identified by peptide phage display and molecular modeling

Myers, M., Davies, J. M., Tong, J. C., Whisstock, J. C., Scealy, M., Mackay, I. R. & Rowley, M. J., 2000, In : Journal of Immunology. p. 3830 - 3838 9 p.

Research output: Contribution to journalArticleResearchpeer-review

460 Citations (Scopus)

Phylogeny of the serpin superfamily: Implications of patterns of amino acid conservation for structure and function

Irving, J., Pike, R. N., Lesk, A. M. & Whisstock, J. C., 2000, In : Genome Research. p. 1845 - 1864 20 p.

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E2 in primary biliary cirrhosis using phage display

Rowley, M. J., Scealy, M., Whisstock, J. C., Jois, J. A., Wijeyewickrema, L. C. & Mackay, I. R., 2000, In : Journal of Immunology. p. 3413 - 3419 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Requirement of leucine-rich repeats of glycoprotein (GP) Ibalpha for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex

Shen, Y., Romo, G. M., Dong, J., Schade, A., McIntire, L. V., Kenny, D., Whisstock, J. C., Berndt, M. C., Lopez, J. A. & Andrews, R. K., 2000, In : Blood. 95, p. 903* - 910 8 p.

Research output: Contribution to journalArticleResearchpeer-review

120 Citations (Scopus)

Requirement of leucine-rich repeats of glycoprotein (GP) Ibα for shear- dependent and static binding of von willebrand factor to the platelet membrane GP Ib-IX-V complex

Shen, Y., Romo, G. M., Dong, J. F., Schade, A., McIntire, L. V., Kenny, D., Whisstock, J. C., Berndt, M. C., López, J. A. & Andrews, R. K., 1 Feb 2000, In : Blood. 95, 3, p. 903-910 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Simple modifications of the serpin reactive site loop convert SCCA2 into a cystein proteinase inhibitor: A critical role for the P3' proline in facilitating RSL cleavage

Luke, C., Schick, C., Tsu, C., Whisstock, J. C., Irving, J., Bromme, D., Juliano, L., Shi, G-P., Chapman, H. A. & Silverman, G. A., 2000, In : Biochemistry. p. 7081 - 7091 11 p.

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Structure and function of the von Willebrand factor A1 domain: analysis with monoclonal antibodies reveals distinct binding sites involved in recognition of the platelet membrane glycoprotein Ib-IX-V complex and ristocetin-dependent activation

De Luca, M., Facey, D. A., Favaloro, E. J., Hertzberg, M. S., Whisstock, J. C., McNally, T., Andrews, R. K. & Berndt, M. C., 2000, In : Blood. p. 164 - 172 9 p.

Research output: Contribution to journalArticleResearchpeer-review

53 Citations (Scopus)
2001

Characterization and functional analysis of Serp3: a novel myxoma virus-encoded serpin invloved in virulence

Guerin, J-L., Gelfi, J., Camus, C., Delverdier, M., Whisstock, J. C., Amardeihl, M-F., Py, R., Bertagnole, S. & Messud-Petit, F., 2001, In : Journal of General Virology. 82, p. 1407-1417 11 p.

Research output: Contribution to journalArticleResearchpeer-review

41 Citations (Scopus)

Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: Identification of a myotubularin-related protein lacking catalytic activity

Nandurkar, H., Caldwell, K. K., Whisstock, J. C., Layton, M. J., Gaudet, E. A., Norris, F. A., Majerus, P. W. & Mitchell, C. A., 2001, In : Proceedings of the National Academy of Sciences. 98, 17, p. 9499 - 9504 6 p.

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)

Importance of the P4' residue in human granzyme B inhibitors and substrates revealed by scanning mutagenesis of the proteinase inhibitor 9 reactive center loop

Sun, J. H., Whisstock, J. C., Harriott, P., Walker, B., Novak, A. J., Thompson, P. E., Smith, A. I. & Bird, P. I., 2001, In : Journal of Biological Chemistry. 276, 18, p. 15177 - 15184 8 p.

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Mutagenesis of the dengue virus type 2 NS3 proteinase and the production of growth-restricted virus

Matusan, A. E., Kelley, P. G., Pryor, M. J., Whisstock, J. C., Davidson, A. D. & Wright, P. J., 2001, In : Journal of General Virology. 82, p. 1647 - 1656 10 p.

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)

Protein structural alignments and functional genomics

Irving, J., Whisstock, J. C. & Lesk, A. M., 2001, In : Proteins: Structure, Function, and Bioinformatics. 42, p. 378 - 382 5 p.

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy

Ludeman, J. P., Whisstock, J. C., Hopkins, P., Le Bonniec, B. & Bottomley, S. P., 2001, In : Biophysical Journal. 80, p. 491 - 497 7 p.

Research output: Contribution to journalArticleResearchpeer-review

The role of strand 1 of the C b-sheet in the structure and function of a1- antitrypsin

Bottomley, S. P., Lawrenson, I. D., Tew, D. J., Dai, W., Whisstock, J. C. & Pike, R. N., 2001, In : Protein Science. 10, p. 2518 - 2524 7 p.

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

The role of strand 1 of the C β-sheet in the structure and function of α1-antitrypsin

Bottomley, S. P., Lawrenson, I. D., Tew, D., Dai, W., Whisstock, J. C. & Pike, R. N., 8 Dec 2001, In : Protein Science. 10, 12, p. 2518-2524 7 p.

Research output: Contribution to journalArticleResearchpeer-review

952 Citations (Scopus)

The serpins are an expanding superfamily of structurally similar but functionally diverse proteins

Silverman, G. A., Bird, P. I., Carrell, R. W., Church, F. C., Coughlin, P. B., Gettins, P. G. W., Irving, J., Lomas, D. A., Luke, C., Moyer, R. W., Pemberton, P. A., Remold-O'Donnell, E., Salvesen, G. S., Travis, J. & Whisstock, J. C., 2001, In : Journal of Biological Chemistry. 276, 36, p. 33293 - 33296 4 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail

Wiradjaja, F., Ooms, L. M., Whisstock, J. C., McColl, B. K., Helfenbaum, L., Sambrook, J., Gething, M. J. & Mitchell, C. A., 2001, In : Journal of Biological Chemistry. 276, 10, p. 7643 - 7653 11 p.

Research output: Contribution to journalArticleResearchpeer-review

2002
51 Citations (Scopus)

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: Engineering α1-antitrypsin to inhibit cathepsin proteases

Irving, J. A., Pike, R. N., Dai, W., Brömme, D., Worrall, D. M., Silverman, G. A., Coetzer, T. H. T., Dennison, C., Bottomley, S. P. & Whisstock, J. C., 16 Apr 2002, In : Biochemistry. 41, 15, p. 4998-5004 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Evidence that serpin architecture intrinsically supports papain-like cystein protease inhibition: Engineering 1- abutrypsin to inhibit acathepsin proteases

Irving, J., Pike, R. N., Dai, W., Bromme, D., Worrall, M. D., Silverman, G. A., Coetzer, T. H., Dennison, C., Bottomley, S. P. & Whisstock, J. C., 2002, In : Biochemistry. 41, 15, p. 4998 - 5004 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Inhibitory activity of a heterchromatin-associated serpin (MENT) against papain like cystein proteinases affects chromatin structure and blocks cell proliferation

Irving, J. A., Shushanov, S., Pike, R. N., Popova, E. Y., Bromme, D., Coetzer, T. H., Bottomley, S. P., Boulynko, I. A., Grigoryev, S. A. & Whisstock, J. C., 2002, In : Journal of Biological Chemistry. 277, 15, p. 13192 - 13201 10 p.

Research output: Contribution to journalArticleResearchpeer-review

79 Citations (Scopus)

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

Irving, J. A., Shushanov, S. S., Pike, R. N., Popova, E. Y., Brömme, D., Coetzer, T. H. T., Bottomley, S. P., Boulynko, I. A., Grigoryev, S. A. & Whisstock, J. C., 12 Apr 2002, In : Journal of Biological Chemistry. 277, 15, p. 13192-13201 10 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Molecular determinants of the mechanisms underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

Quinsey, N. S., Whisstock, J. C., Le Bonniec, B., Louvain, V., Bottomley, S. P. & Pike, R. N., 2002, In : Journal of Biological Chemistry. 277, 18, p. 15971 - 15978 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Molecular Modeling of the Seven Tandem Leucine-Rich Repeats within the Ligand-Binding Region of Platelet Glycoprotein Ib

Whisstock, J. C., Shen, Y., Lopez, J. A., Andrews, R. K. & Berndt, M. C., 2002, In : Thrombosis and Haemostasis. 87, 2, p. 329 - 333 5 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Molecular modeling of the seven tandem leucine-rich repeats within the ligand-binding region of platelet glycoprotein Ibα

Whisstock, J. C., Shen, Y., López, J. A., Andrews, R. K. & Berndt, M. C., 21 Feb 2002, In : Thrombosis and Haemostasis. 87, 2, p. 329-333 5 p.

Research output: Contribution to journalArticleResearchpeer-review

40 Citations (Scopus)

Probing the role of the F-Helix in serpin stability through a single tryptophan substitution

Cabrita, L., Whisstock, J. C. & Bottomley, S. P., 2002, In : Biochemistry. 41, 14, p. 4575 - 4581 7 p.

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Serpins: finely balanced conformational traps

Pike, R. N., Bottomley, S. P., Irving, J., Bird, P. I. & Whisstock, J. C., 2002, In : IUBMB Life. 54, 1, p. 1 - 7 7 p.

Research output: Contribution to journalArticleResearchpeer-review

93 Citations (Scopus)

Serpins in Prokaryotes

Irving, J., Steenbakkers, P. J., Lesk, A. M., Op Den Camp, H. J., Pike, R. N. & Whisstock, J. C., 2002, In : Molecular Biology and Evolution. 19, 11, p. 1881 - 1890 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Subunit fusion are functionally incorporated into mitochondrial F1F0 synthase arguing against a rigid cap structure at the top of F1

Prescott, M., Nowakowski, S., Gavin, P., Nagley, P., Whisstock, J. C. & Devenish, R. J., 2002, In : Journal of Biological Chemistry. 278, 1, p. 251 - 256 6 p.

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionizatin Mass Spectronometry Techniques Based on Isotope Exchange Effects

Hearn, M. T. W., Quirino, J. P., Whisstock, J. C. & Terabe, S., 2002, In : Analytical Chemistry. 74, 6, p. 1467 - 1475 9 p.

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)

The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases

Al-Khunaizi, M., Luke, C. J., Askew, Y. S., Pak, S. C., Cataltepe, S., Miller, D. R., Mills, D. R., Tsu, C., Bromme, D., Irving, J., Whisstock, J. C. & Silverman, G. A., 2002, In : Biochemistry. 41, 9, p. 3189 - 3199 11 p.

Research output: Contribution to journalArticleResearchpeer-review

The structure and function of catalytic domains within inositol polyphosphate 5- phosphates

Whisstock, J. C., Wiradjaja, F., Waters, J. & Gurung, R., 2002, In : IUBMB Life. 53, 1, p. 15 - 23 9 p.

Research output: Contribution to journalArticleResearchpeer-review

2003
273 Citations (Scopus)

A structural basis for the selection of dominant T cell receptors in antiviral immunity

Kjer-Nielsen, L., Clements, C. S., Purcell, A. W., Brooks, A. G., Whisstock, J. C., Burrows, S. R., McCluskey, J. & Rossjohn, J., 2003, In : Immunity. 18, 1, p. 53 - 64 12 p.

Research output: Contribution to journalArticleResearchpeer-review