Projects per year
My research focuses on the molecular interactions that take place in human cells in human and viral RNA biology, innate immunity and cellular signalling.
The laboratory, run jointly with A/Prof Matthew Wilce, aims to better understand molecular recognition by determining the overall shapes, as well as the precise three-dimensional structures of macromolecular complexes and by testing their affinity and specificity using biophysical techniques. We investigate several molecular systems involved in RNA biology, innate immunity and cancer cell progression. These include the study of proteins which bind to mRNA and regulate translation, proteins which recognise RNA in innate immunity and the development of peptides which inhibit cancer cell proliferation and migration.
Current Research Projects
Protein-RNA interactions in antiviral cellular defence and inflammation
Protein-RNA interactions are integral to cellular biology – both in normal cellular function and also in cells subject to the stresses of viral invasion. Proteins are responsible for the detection of viral RNA, and initiation of the innate immune response. Proteins direct post-transcriptional regulation of cytokines produced as a result of cellular stress, and are responsible for preventing their over-expression. In some cases, cellular proteins that normally function in translational control are hijacked by viral RNA as part of the viral mechanism of replication in the cell. Underlying each of these types molecular events are intricate and specialised molecular interactions. Their understanding would greatly advance our knowledge of antiviral cellular defence and potentially lead to new means to combat virus-related disease and inflammatory disorders.
Our lab has specialised in the study of protein-RNA interactions, using biophysical and structural tools to better understand the basis for their affinity, specificity and conformational consequences underlying their mechanism of action. Our objective is to delineate specific protein-RNA systems relevant to antiviral cellular defence.
I completed a B. Sc (Hons) degree at Monash University, specialising in novel peptide chemistries at honours level. In 1994 I graduated with a Ph.D. in Medicinal Chemistry from the Victorian College of Pharmacy (now MIPS, Monash University) in the field of NMR structural and dynamics studies of peptides and proteins. I then took up a position at Queensland University in the Centre for Drug Design and Development with Professor David Craik, synthesising proteins using peptide and novel linkage chemistries. I then spent several years at the University of Sydney with Professor Glenn King, extending my expertise to include molecular biological and protein expression and purification techniques as well as 3D heteronuclear NMR spectroscopy and analytical ultracentrifugation. Here I began to develop an interest in structural and dynamic studies of novel cancer targets as well as the use of peptides for transporting bioactive compounds across cell walls. I also embarked on structural studies of a protein:DNA complex involved in replication fork arrest for which I was awarded an ARC Postdoctoral Fellowship. In 1999 I established my own laboratory as a joint member of Biochemistry and Chemistry at the Department at the University of Western Australia, where I extended my research into the field of structural and dynamic studies of protein-RNA interactions involved in mRNA stabilization in collaboration with partner, Prof Matthew Wilce. In 2001 I was awarded an ARC Fellowship to pursue molecular interactions studies, and have added surface plasmon resonance and isothermal titration calorimetry to the repertoire of biophysical techniques that underpins the work. In 2005 I moved with Matthew Wilce to Monash University to establish labs in the dynamic Department of Biochemistry and Molecular Biology. I was appointed as a Senior lecturer in 2007 and promoted to Associate Professor in 2010. I now engage in both teaching and research in the macromolecular sciences.
Monash teaching commitment
I currently teach into the following units:
BMS1011, BCH3031, BMS3031, BMS2062, BCH3052
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Research area keywords
- Protein-RNA Interactions
- Structural Biology
- Protein Chemistry
- Cancer And Immunity
Dive into details
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Wilce, J., Fox, A. & Fletcher, S.
1/01/22 → 31/12/22
1/01/19 → 31/12/22
Dunstone, M., Whisstock, J., Daly, R., Atkinson, S., Turner, S., Lithgow, T., Gras, S., Stone, M., Davidovich, C., Rood, J., Coulibaly, F., Wilce, J., Borg, N., McGowan, S., Buckle, A., Elmlund, D., Elmlund, H., Belousoff, M., Ellisdon, A., Law, R., Purcell, A., Cryle, M., Nold, M., Nold, C., Warr, C., Johnson, T., Henstridge, M., Aguilar, M., Stroud, D. & Ryan, M.
1/12/16 → 31/12/17
1/01/16 → 31/12/18
Loughlin, F. E., West, D. L., Gunzburg, M. J., Waris, S., Crawford, S. A., Wilce, M. C. J. & Wilce, J. A., 18 Mar 2021, In: Nucleic Acids Research. 49, 5, p. 2403-2417 15 p.
Research output: Contribution to journal › Article › Research › peer-reviewOpen Access
Comparison between clickable cyclic TAT and penetratin for delivery of cyclic and bicyclic-peptide cargosKulkarni, K., Sang, J., Ma, X. & Wilce, J. A., Jul 2020, In: Peptide Science. 112, 4, 11 p., e24163.
Research output: Contribution to journal › Article › Research › peer-review
Detailed protocol for optimised expression and purification of functional monomeric human Heat Shock Factor 1Polidano, J., Vankadari, N., Price, J. T. & Wilce, J. A., Dec 2020, In: Protein Expression and Purification. 176, 9 p., 105722.
Research output: Contribution to journal › Article › Other › peer-review
Watson, G. M. & Wilce, J. A., 2 Feb 2020, In: International Journal of Molecular Sciences. 21, 4, 10 p., 1336.
Research output: Contribution to journal › Article › Research › peer-reviewOpen AccessFile
Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26Vankadari, N. & Wilce, J. A., 17 Mar 2020, In: Emerging Microbes & Infections. 9, 1, p. 601-604 4 p.
Research output: Contribution to journal › Letter › Research › peer-reviewOpen AccessFile