Christian Nefzger

Dr

20062019

Research output per year

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Research Output

2019

Class-Switch Recombination Occurs Infrequently in Germinal Centers

Roco, J. A., Mesin, L., Binder, S. C., Nefzger, C., Gonzalez-Figueroa, P., Canete, P. F., Ellyard, J., Shen, Q., Robert, P. A., Cappello, J., Vohra, H., Zhang, Y., Nowosad, C. R., Schiepers, A., Corcoran, L. M., Toellner, K. M., Polo, J. M., Meyer-Hermann, M., Victora, G. D. & Vinuesa, C. G., 20 Aug 2019, In : Immunity. 51, 2, p. 337-350 14 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)
Open Access
File
2 Citations (Scopus)

Generation of mouse-induced pluripotent stem cells by lentiviral transduction

Liu, X., Chen, J., Firas, J., Paynter, J. M., Nefzger, C. M. & Polo, J. M., 2019, Mouse Cell Culture: Methods and Protocols. Bertoncello, I. (ed.). New York, NY: Humana Press, p. 63-76 14 p. (Methods in Molecular Biology; vol. 1940).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Production of high-titer lentiviral particles for stable genetic modification of mammalian cells

Larcombe, M. R., Manent, J., Chen, J., Mishra, K., Liu, X. & Nefzger, C. M., 1 Jan 2019, Mouse Cell Culture: Methods and Protocols. Bertoncello, I. (ed.). New York: Humana Press, p. 47-61 15 p. (Methods in Molecular Biology; vol. 1940).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network

Seruggia, D., Oti, M., Tripathi, P., Canver, M. C., LeBlanc, L., Di Giammartino, D. C., Bullen, M. J., Nefzger, C. M., Sun, Y. B. Y., Farouni, R., Polo, J. M., Pinello, L., Apostolou, E., Kim, J., Orkin, S. H. & Das, P. P., 20 Jun 2019, In : Molecular Cell. 74, 6, p. 1148-1163 16 p.

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)
2018

A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs

Pflueger, C., Tan, D., Swain, T., Nguyen, T., Pflueger, J., Nefzger, C., Polo, J. M., Ford, E. & Lister, R., 1 Aug 2018, In : Genome Research. 28, 8, p. 1193-1206 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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27 Citations (Scopus)

Fine Tuning of Canonical Wnt Stimulation Enhances Differentiation of Pluripotent Stem Cells Independent of β-Catenin-Mediated T-Cell Factor Signaling

Chen, J., Nefzger, C. M., Rossello, F., Sun, Y. B. Y., Lim, S. M., Liu, X., De Boer, S., Knaupp, A. S., Li, J., Davidson, K. C., Polo, J. M. & Barberi, T., 1 Jun 2018, In : Stem Cells. 36, 6, p. 822-833 12 p.

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Identification of dynamic undifferentiated cell states within the male germline

La, H. M., Mäkelä, J-A., Chan, A-L., Rossello, F. J., Nefzger, C. M., Legrand, J. M. D., De Seram, M., Polo, J. M. & Hobbs, R. M., 19 Jul 2018, In : Nature Communications. 9, 1, 18 p., 2819.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
18 Citations (Scopus)

Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks

Gazina, E. V., Morrisroe, E., Mendis, G. D. C., Michalska, A. E., Chen, J., Nefzger, C. M., Rollo, B. N., Reid, C. A., Pera, M. F. & Petrou, S., 1 Jan 2018, In : Journal of Neuroscience Methods. 293, p. 53-58 6 p.

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

SRSF3 promotes pluripotency through nanog mRNA export and coordination of the pluripotency gene expression program

Ratnadiwakara, M., Archer, S. K., Dent, C. I., Ruiz De Los Mozos, I., Beilharz, T. H., Knaupp, A. S., Nefzger, C. M., Polo, J. M. & Anko, M-L., 9 May 2018, In : eLife. 7, 28 p., e37419.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
8 Citations (Scopus)
2017

Cell Type of Origin Dictates the Route to Pluripotency

Nefzger, C. M., Rossello, F. J., Chen, J., Liu, X., Knaupp, A. S., Firas, J., Paynter, J. M., Pflueger, J., Buckberry, S., Lim, S. M., Williams, B., Alaei, S., Faye-Chauhan, K., Petretto, E., Nilsson, S. K., Lister, R., Ramialison, M., Powell, D. R., Rackham, O. J. L. & Polo, J. M., 5 Dec 2017, In : Cell Reports. 21, 10, p. 2649-2660 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
19 Citations (Scopus)

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Liu, X., Nefzger, C. M., Rossello, F. J., Chen, J., Knaupp, A. S., Firas, J., Ford, E., Pflueger, J., Paynter, J. M., Chy, H. S., O'Brien, C. M., Huang, C., Mishra, K., Hodgson-Garms, M., Jansz, N., Williams, S. M., Blewitt, M. E., Nilsson, S. K., Schittenhelm, R. B., Laslett, A. L. & 2 others, Lister, R. & Polo, J. M., 31 Oct 2017, In : Nature Methods. 14, 11, p. 1055-1062 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
32 Citations (Scopus)

DEAD-Box RNA Binding Protein DDX5: Not a Black-Box during Reprogramming

Nefzger, C. & Polo, J. M., 6 Apr 2017, In : Cell Stem Cell. 20, 4, p. 419-420 2 p.

Research output: Contribution to journalShort SurveyOtherpeer-review

2 Citations (Scopus)

Gut microbial metabolites limit the frequency of autoimmune T cells and protect against type 1 diabetes

Marino, E., Richards, J. L., McLeod, K. H., Stanley, D., Yap, Y. A., Knight, J., McKenzie, C., Kranich, J., Oliveira, A. C., Rossello, F. J., Krishnamurthy, B., Nefzger, C. M., Macia, L., Thorburn, A., Baxter, A. G., Morahan, G., Wong, L. H., Polo, J. M., Moore, R. J., Lockett, T. J. & 4 others, Clarke, J. M., Topping, D. L., Harrison, L. C. & Mackay, C. R., May 2017, In : Nature Immunology. 18, 5, p. 552-562 13 p.

Research output: Contribution to journalArticleResearchpeer-review

178 Citations (Scopus)

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

O'Brien, C. M., Chy, H. S., Zhou, Q., Blumenfeld, S., Lambshead, J. W., Liu, X., Kie, J., Capaldo, B. D., Chung, T-L., Adams, T. E., Phan, T., Bentley, J. D., McKinstry, W. J., Oliva, K., McMurrick, P. J., Wang, Y-C., Rossello, F. J., Lindeman, G. J., Chen, D., Jarde, T. & 7 others, Clark, A. T., Abud, H. E., Visvader, J. E., Nefzger, C. M., Polo, J. M., Loring, J. F. & Laslett, A. L., 1 Mar 2017, In : Stem Cells. 35, 3, p. 626-640 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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10 Citations (Scopus)

Transient and Permanent Reconfiguration of Chromatin and Transcription Factor Occupancy Drive Reprogramming

Knaupp, A. S., Buckberry, S., Pflueger, J., Lim, S. M., Ford, E., Larcombe, M. R., Rossello, F. J., de Mendoza, A., Alaei, S., Firas, J., Holmes, M. L., Nair, S. S., Clark, S. J., Nefzger, C. M., Lister, R. & Polo, J. M., 7 Dec 2017, In : Cell Stem Cell. 21, 6, p. 834-845.e6 18 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
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36 Citations (Scopus)
2016

An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3

Alaei, S., Knaupp, A. S., Lim, S. M., Chen, J., Holmes, M. L., Ankö, M-L., Nefzger, C. M. & Polo, J. M., 1 Jul 2016, In : Stem Cell Research. 17, 1, p. 49-53 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
4 Citations (Scopus)

A predictive computational framework for direct reprogramming between human cell types

Rackham, O. J. L., Firas, J., Fang, H., Oates, M. E., Holmes, M. L., Knaupp, A. S., The FANTOM Consortium, Suzuki, H., Nefzger, C. M., Daub, C. O., Shin, J. W., Petretto, E., Forrest, A. R. R., Hayashizaki, Y., Polo, J. M. & Gough, J., 18 Jan 2016, In : Nature Genetics. 48, 3, p. 331-335 5 p.

Research output: Contribution to journalLetterOtherpeer-review

102 Citations (Scopus)

A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells

Nefzger, C. M., Jarde, T., Rossello, F. J., Horvay, K., Knaupp, A. S., Powell, D. R., Chen, J. Y., Abud, H. E. & Polo, J. M., 2016, In : Stem Cell Reports. 6, 3, p. 321 - 329 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
11 Citations (Scopus)
2015

Isolation of reprogramming intermediates during generation of induced pluripotent stem cells from mouse embryonic fibroblasts

Nefzger, C. M., Alaei Shehni, S. & Polo, J. M., 2015, Cell Reprogramming: Methods and Protocols. Verma, P. J. & Sumer, H. (eds.). 1st ed. Totowa NJ USA: Humana Press, p. 205 - 218 14 p. (Methods in Molecular Biology: Springer Protocols; vol. 1330).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium

Horvay, K., Jarde, T., Casagranda, F., Perreau, V. M., Haigh, K., Nefzger, C. M., Akhtar, R., Gridley, T., Berx, G., Haigh, J. J., Barker, N., Polo, J. M., Hime, G. R. & Abud, H. E., 2015, In : The EMBO Journal. 34, 10, p. 1319 - 1335 17 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
25 Citations (Scopus)
2014

Cell surface marker mediated purification of iPS cell intermediates from a reprogrammable mouse model

Nefzger, C. M., Alaei Shehni, S., Knaupp, A. S., Holmes, M. L. & Polo, J. M., 2014, In : Journal of Visualized Experiments. 91, (Art. No.: e51728), p. 1 - 8 8 p.

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

GM-CSF and MEF-conditioned media support feeder-free reprogramming of mouse granulocytes to iPS cells

Firas, J., Liu, E. X., Nefzger, C. & Polo, J. M., 2014, In : Differentiation. 87, 5, p. 193 - 199 7 p.

Research output: Contribution to journalComment / DebateResearchpeer-review

7 Citations (Scopus)
2013

Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells

Kelly, R. D. W., Rodda, A. E., Dickinson, A., Mahmud, A., Nefzger, C., Lee, W., Forsythe, J. S., Polo, J. M., Trounce, I. A., McKenzie, M., Nisbet, D. R. & St John, J. C., 2013, In : Stem Cells. 31, 4, p. 703 - 716 14 p.

Research output: Contribution to journalArticleResearchpeer-review

51 Citations (Scopus)

Probing transcription factor diffusion dynamics in the living mammalian embryo with photoactivatable fluorescence correlation spectroscopy

Kaur, G., Costa, M., Nefzger, C., Silva, J., Fierro-Gonzalez, J. C., Polo, J. M., Bell, T. D. M. & Plachta, N., 2013, In : Nature Communications. 4, 1637, p. 1 - 13 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
52 Citations (Scopus)
2012

A molecular roadmap of reprogramming somatic cells into iPS cells

Polo, J. M., Anderssen, E., Walsh, R. M., Schwarz, B. A., Nefzger, C., Lim, S. M., Borkent, M., Apostolou, E., Alaei Shehni, S., Cloutier, J., Bar-Nur, O., Cheloufi, S., Stadtfeld, M., Figueroa, M. E., Robinton, D., Natesan, S., Melnick, A., Zhu, J., Ramaswamy, S. & Hochedlinger, K., 2012, In : Cell. 151, 7, p. 1617 - 1632 16 p.

Research output: Contribution to journalArticleResearchpeer-review

507 Citations (Scopus)

Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells

Nefzger, C., Su, C. T. E., Fabb, S. A., Hartley, B. J., Zeng, W., Haynes, J. M. & Pouton, C. W., 2012, In : Stem Cells. 30, 7, p. 1349 - 1361 13 p.

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)
2011
18 Citations (Scopus)

Midbrain and forebrain patterning delivers immunocytochemically and functionally similar populations of neuropeptide Y containing GABAergic neurons

Khaira, S., Nefzger, C., Beh, S., Pouton, C. & Haynes, J., 2011, In : Neurochemistry International. 59, 3, p. 413 - 420 8 p.

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)