Ashley Buckle

Assoc Professor

Accepting PhD Students

PhD projects

Engineering proteins for therapeutic and diagnostic use

1993 …2020

Research activity per year

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Personal profile

Research interests

Ashley Buckle has more than three decades of expertise in structural biology and protein engineering, with a focus on combining state-of-the-art protein biochemistry, biophysical, structural and computational approaches in protein engineering programs. Ashley trained at the MRC Centre for Protein Engineering in Cambridge, under the supervision of Sir Alan Fersht, a founder and pioneer of protein engineering. He has published influential research in diverse areas of protein science, and has made major contributions to protein structure/folding/function, protein design and engineering, bioinformatics, molecular dynamics, immunology, and protease biology. He has consistently published in the best journals, which include SciencePNAS (10), Nature Struct. Mol. Biol. (2), Nature Chem. Biol., Nature Communications and Nature Immunology (2). He has published 144 papers in total, with an h-index of 45 and more than 7000 citations. He has secured in excess of AUD$13 million in research funding and is the named co-inventor on 8 patents. Career highlights include the co-invention of minichaperone protein refolding technology (PNAS x2 1997), leading to MRC spin out Avidis; discovery of mechanisms of autoinactivation and antibody recognition of GAD65, an autoantigen in type 1 diabetes and regulator of neurotransmitter homeostasis (Nature S&MB 2007, PNAS 2014); design of hyperstable non-antibody scaffolds for therapeutic and diagnostic use (PEDS 2015, 2016 ); creation of new biologics for augmentation therapy for antitrypsin deficiency-related emphysema; and design of novel multi-specific immunoglobulin-like molecules. Several projects are currently involved in commercialisation activities. 

Bioinformatics databases we have developed:

D2Odb

REFOLD

PolyQ

KinetochoreDB

MyTardis

Store.Synchrotron

Mustang-MR

 

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No longer maintained: Department pageLab Facebook page

Biography

I completed my PhD in 1994 in the laboratory of Prof Sir Alan Fersht at the University of Cambridge, on the structure determination of protein-DNA and protein-protein complexes. As a postdoc then staff scientist at the MRC Centre Cambridge, I made contributions to the understanding of protein stability, molecular recognition and the action of molecular chaperones. I relocated to Monash University, Australia in 2003 and am currently a group leader in the Department of Biochemistry and Molecular Biology

 A longer bio is here.

 

External positions

CEO & Founder, PTNG Consulting

1 Jan 2020 → …

Research area keywords

  • Protein design
  • Protein Biophysics
  • Protein folding
  • Protein engineering
  • Molecular Dynamics
  • Directed evolution

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