Ashley Buckle

Personal profile

Research interests

We combine x-ray crystallography and biophysics with molecular simulation to study the structure, folding and dynamics of proteins, with a particular focus on the design and engineering of proteins for medical and biotechnological application. Our team is a unique and exciting mix of experimentalists and computational biologists using modeling and simulation to make predictions that can be tested in the lab.

Bioinformatics databases we have developed:

Department page | All publications on PubMed | Lab Facebook page | ORCID | Google Scholar

Biography

I completed my PhD in 1994 in the laboratory of Prof Sir Alan Fersht at the University of Cambridge, on the structure determination of protein-DNA and protein-protein complexes. As a postdoc then staff scientist at the MRC Centre Cambridge, I made contributions to the understanding of protein stability, molecular recognition and the action of molecular chaperones. I relocated to Monash University, Australia in 2003 and am currently a group leader in the Department of Biochemistry and Molecular Biology.

A longer bio is here.

Research area keywords

Protein design
Protein Biophysics
Protein folding
Protein engineering
Molecular Dynamics
Directed evolution

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Projects 2004 2019

1 Active
14 Finished

Design and engineering of adnectins for diagnosis and therapy

Buckle, A. & Jackson, C.

National Health & Medical Research Council (NHMRC)

1/01/17 → 31/12/19

Project: Research

Engineering α-1 antitrypsin for augmentation therapy

Buckle, A.

Alpha-1 Foundation

1/07/17 → 30/06/18

Project: Research

Beckman Coulter Optima Analytical Ultracentrifuge (AUC)

Dunstone, M., Whisstock, J., Daly, R., Atkinson, S., Turner, S., Lithgow, T., Gras, S., Stone, M., Davidovich, C., Rood, J., Coulibaly, F., Wilce, J., Borg, N., McGowan, S., Buckle, A., Elmlund, D., Elmlund, H., Belousoff, M., Ellisdon, A., Law, R., Purcell, A., Cryle, M., Nold, M., Nold, C., Warr, C., Johnson, T., Henstridge, M., Aguilar, M., Stroud, D. & Ryan, M.

1/12/16 → 31/12/17

Project: Research

Investigating the dynamic nature of antibody stability

Buckle, A.

Australian Research Council (ARC)

1/01/16 → 30/12/18

Project: Research

Design and engineering of proteins for biotechnology and biomedicine

Buckle, A.

Australian Research Council (ARC), Monash University

1/01/15 → 24/12/18

Project: Research

Research Output 1993 2020

116 Article
5 Chapter (Book)
5 Conference Paper
5 Review Article
2 More
- 1 Abstract
- 1 Letter

Strategies for Increasing Protein Stability

Chandler, P. G., Broendum, S. S., Riley, B. T., Spence, M. A., Jackson, C. J., McGowan, S. & Buckle, A. M., 2020, Protein Nanotechnology: Protocols, Instrumentation, and Applications. Gerrard, J. A. & Domigan, L. J. (eds.). New York, NY: Humana Press, p. 163-181 19 p. (Methods in Molecular Biology; vol. 2073).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Crystal structure of the inhibitor-free form of the serine protease kallikrein-4

Riley, B. T., Hoke, D. E., McGowan, S. & Buckle, A. M., Aug 2019, In : Acta Crystallographica Section F: Structural Biology Communications. F75, p. 543-546 4 p.

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity

Zammit, N. W., Siggs, O. M., Gray, P. E., Horikawa, K., Langley, D. B., Walters, S. N., Daley, S. R., Loetsch, C., Warren, J., Yap, J. Y., Cultrone, D., Russell, A., Malle, E. K., Villanueva, J. E., Cowley, M. J., Gayevskiy, V., Dinger, M. E., Brink, R., Zahra, D., Chaudhri, G. & 36 others, Karupiah, G., Whittle, B., Roots, C., Bertram, E., Yamada, M., Jeelall, Y., Enders, A., Clifton, B. E., Mabbitt, P. D., Jackson, C. J., Watson, S. R., Jenne, C. N., Lanier, L. L., Wiltshire, T., Spitzer, M. H., Nolan, G. P., Schmitz, F., Aderem, A., Porebski, B. T., Buckle, A. M., Abbott, D. W., Ziegler, J. B., Craig, M. E., Benitez-Aguirre, P., Teo, J., Tangye, S. G., King, C., Wong, M., Cox, M. P., Phung, W., Tang, J., Sandoval, W., Wertz, I. E., Christ, D., Goodnow, C. C. & Grey, S. T., Oct 2019, In : Nature Immunology. 20, 10, p. 1299-1310 12 p.

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

KLK4 Inhibition by Cyclic and Acyclic Peptides: Structural and Dynamical Insights into Standard-Mechanism Protease Inhibitors

Riley, B. T., Ilyichova, O., De Veer, S. J., Swedberg, J. E., Wilson, E., Hoke, D. E., Harris, J. M. & Buckle, A. M., 28 May 2019, In : Biochemistry. 58, 21, p. 2524-2533 10 p.

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Potent, multi-target serine protease inhibition achieved by a simplified β-sheet motif

Chen, X., Riley, B. T., De Veer, S. J., Hoke, D. E., Van Haeften, J., Leahy, D., Swedberg, J. E., Brattsand, M., Hartfield, P. J., Buckle, A. M. & Harris, J. M., 22 Jan 2019, In : PLoS ONE. 14, 1, 15 p., e0210842.

Research output: Contribution to journal › Article › Research › peer-review

Open Access

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