Personal profile

Research interests

We combine x-ray crystallography and biophysics with molecular simulation to study the structure, folding and dynamics of proteins, with a particular focus on the design and engineering of proteins for medical and biotechnological application. Our team is a unique and exciting mix of experimentalists and computational biologists using modeling and simulation to make predictions that can be tested in the lab.

Bioinformatics databases we have developed:

REFOLD

Mustang-MR

PolyQ

KinetochoreDB

MyTardis

Store.Synchrotron

 

Department pageAll publications on PubMed | Lab Facebook page | ORCID | Google Scholar

Biography

I completed my PhD in 1994 in the laboratory of Prof Sir Alan Fersht at the University of Cambridge, on the structure determination of protein-DNA and protein-protein complexes. As a postdoc then staff scientist at the MRC Centre Cambridge, I made contributions to the understanding of protein stability, molecular recognition and the action of molecular chaperones. I relocated to Monash University, Australia in 2003 and am currently a group leader in the Department of Biochemistry and Molecular Biology

 A longer bio is here.

 

Keywords

  • Protein design
  • Protein Biophysics
  • Protein folding
  • Protein engineering
  • Molecular Dynamics
  • Directed evolution

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Projects 2004 2019

Research Output 1993 2017

REFOLDdb: A new and sustainable gateway to experimental protocols for protein refolding

Mizutani, H., Sugawara, H., Buckle, A. M., Sangawa, T., Miyazono, K. I., Ohtsuka, J., Nagata, K., Shojima, T., Nosaki, S., Xu, Y., Wang, D., Hu, X., Tanokura, M. & Yura, K. 24 Apr 2017 In : BMC Structural Biology. 17, 1, 4

Research output: Research - peer-reviewArticle

Open Access
File

Structural reconstruction of protein ancestry

Rouet, R., Langley, D. B., Schofield, P., Christie, M., Roome, B., Porebski, B. T., Buckle, A. M., Clifton, B. E., Jackson, C. J., Stock, D. & Christ, D. 11 Apr 2017 In : Proceedings of the National Academy of Sciences. 114, 15, p. 3897-3902 6 p.

Research output: Research - peer-reviewArticle

The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger

Keightley, M-C., Carradice, D. P., Layton, J. E., Pase, L., Bertrand, J. Y., Wittig, J. G., Dakic, A., Badrock, A. P., Cole, N. J., Traver, D., Nutt, S. L., McCoey, J., Buckle, A. M., Heath, J. K. & Lieschke, G. J. 6 Apr 2017 In : Nature Communications. 8, 13 p., 14911

Research output: Research - peer-reviewArticle

Open Access
File

A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation

Pharo, E. A., Cane, K. N., McCoey, J., Buckle, A. M., Oosthuizen, W. H., Guinet, C. & Arnould, J. P. Y. 1 Mar 2016 In : Gene. 578, 1, p. 7-16 10 p.

Research output: Research - peer-reviewArticle

Circumventing the stability-function trade-off in an engineered FN3 domain

Porebski, B. T., Conroy, P. J., Drinkwater, N., Schofield, P., Vazquez-Lombardi, R., Hunter, M. R., Hoke, D. E., Christ, D., McGowan, S. & Buckle, A. M. 1 Nov 2016 In : Protein Engineering, Design and Selection. 29, 11, p. 541-550 9 p.

Research output: Research - peer-reviewArticle

Press / Media

The inaugural Monash Biomedicine Discovery Institute (BDI) Industry Showcase event

Ashley Maurice Buckle & Peter Geoffrey Chandler

15/11/17

1 item of media coverage

Press / Media: Research

'Jekyll and Hyde' protein linked to type 1 diabetes

Ashley Maurice Buckle

10/06/14

1 item of media coverage

Press / Media: Research

A reliable TARDIS for precious research data

Ashley Maurice Buckle

3/04/13

1 item of media coverage

Press / Media: Research

Sewer to saviour, PlyC is back

Ashley Maurice Buckle & Sheena McGowan

1/02/13

1 item of media coverage

Press / Media: Research