Projects per year
Personal profile
Research interests
We combine x-ray crystallography and biophysics with molecular simulation to study the structure, folding and dynamics of proteins, with a particular focus on the design and engineering of proteins for medical and biotechnological application. Our team is a unique and exciting mix of experimentalists and computational biologists using modeling and simulation to make predictions that can be tested in the lab.
Bioinformatics databases we have developed:
Department page | All publications on PubMed | Lab Facebook page | ORCID | Google Scholar
Biography
I completed my PhD in 1994 in the laboratory of Prof Sir Alan Fersht at the University of Cambridge, on the structure determination of protein-DNA and protein-protein complexes. As a postdoc then staff scientist at the MRC Centre Cambridge, I made contributions to the understanding of protein stability, molecular recognition and the action of molecular chaperones. I relocated to Monash University, Australia in 2003 and am currently a group leader in the Department of Biochemistry and Molecular Biology.
A longer bio is here.
Keywords
- Protein design
- Protein Biophysics
- Protein folding
- Protein engineering
- Molecular Dynamics
- Directed evolution
Network
Recent external collaboration on country level. Dive into details by clicking on the dots.
Projects 2004 2019
Design and engineering of adnectins for diagnosis and therapy
Buckle, A. & Jackson, C.
National Health & Medical Research Council (NHMRC)
1/01/17 → 31/12/19
Project: Research
Beckman Coulter Optima Analytical Ultracentrifuge (AUC)
Dunstone, M., Whisstock, J., Daly, R., Atkinson, S., Turner, S., Lithgow, T., Gras, S., Stone, M., Davidovich, C., Rood, J., Coulibaly, F., Wilce, J., Borg, N., McGowan, S., Buckle, A., Elmlund, D., Elmlund, H., Belousoff, M., Ellisdon, A., Law, R., Purcell, A., Cryle, M., Nold, M., Nold, C., Warr, C., Johnson, T., Henstridge, M., Aguilar, M., Stroud, D. & Ryan, M.
1/12/16 → 31/12/17
Project: Research
Investigating the dynamic nature of antibody stability
Australian Research Council (ARC)
1/01/16 → 30/12/18
Project: Research
Design and engineering of proteins for biotechnology and biomedicine
Australian Research Council (ARC), Monash University
1/01/15 → 24/12/18
Project: Research
Research Output 1993 2019
KLK4 Inhibition by Cyclic and Acyclic Peptides: Structural and Dynamical Insights into Standard-Mechanism Protease Inhibitors
Riley, B. T., Ilyichova, O., De Veer, S. J., Swedberg, J. E., Wilson, E., Hoke, D. E., Harris, J. M. & Buckle, A. M., 28 May 2019, In : Biochemistry. 58, 21, p. 2524-2533 10 p.Research output: Contribution to journal › Article › Research › peer-review
Potent, multi-target serine protease inhibition achieved by a simplified β-sheet motif
Chen, X., Riley, B. T., De Veer, S. J., Hoke, D. E., Van Haeften, J., Leahy, D., Swedberg, J. E., Brattsand, M., Hartfield, P. J., Buckle, A. M. & Harris, J. M., 22 Jan 2019, In : PLoS ONE. 14, 1, 15 p., e0210842.Research output: Contribution to journal › Article › Research › peer-review
Open Access
File
Prostate cancer risk-associated single-nucleotide polymorphism affects prostate-specific antigen glycosylation and its function
Srinivasan, S., Stephens, C., Wilson, E., Panchadsaram, J., DeVoss, K., Koistinen, H., Stenman, U. H., Brook, M. N., on behalf of the PRACTICAL consortium, Buckle, A. M., Klein, R. J., Lilja, H., Clements, J. & Batra, J., 1 Jan 2019, In : Clinical Chemistry. 65, 1, p. E1-E9 9 p.Research output: Contribution to journal › Article › Research › peer-review
Protein engineering: the potential of remote mutations
Wilding, M., Hong, N., Spence, M., Buckle, A. M. & Jackson, C. J., Apr 2019, In : Biochemical Society Transactions. 47, 2, p. 701-711 11 p.Research output: Contribution to journal › Review Article › Research › peer-review
Reactive centre loop dynamics and serpin specificity
Marijanovic, E. M., Fodor, J., Riley, B. T., Porebski, B. T., Costa, M. G. S., Kass, I., Hoke, D. E., McGowan, S. & Buckle, A. M., 7 Mar 2019, In : Scientific Reports. 9, 1, 15 p., 3870.Research output: Contribution to journal › Article › Research › peer-review
Open Access
File
Press / Media
Creating order from disorder: uncovering the origins of protein evolution
22/01/19
1 media contribution
Press/Media: Article/Feature
The spark that created life
12/09/18
1 item of media coverage
Press/Media: Blogs, Podcasts and Social Media › Social Media Article
The spark that created life
12/09/18
1 item of media coverage
Press/Media: Blogs, Podcasts and Social Media › Social Media Article
The inaugural Monash Biomedicine Discovery Institute (BDI) Industry Showcase event
Ashley Buckle & Peter Geoffrey Chandler
15/11/17
1 item of media coverage
Press/Media: Research