Projects per year
Personal profile
Biography
I received a BSc (2008) from the University of Melbourne, before moving to Monash University to complete an M.BSc(part 1) (2009) and PhD (2016) under the supervision of A/Prof Travis Beddoe and Prof. Jamie Rossjohn. My postgraduate research into the structural and functional characterisation of novel enzymes in Mycobacterium tuberculosis provided me a solid biochemical skillset. Upon completion, I have been employed by The Department of Biochemistry and Molecular Biology, Monash University, since 2014. Here, my research has focused on the field of structural immunology, where I aim to understand the critical events in lipid-mediated immunity in the context of autoreactivity and bacterial infection. Through my research, I employ a complementary set of biochemical, cellular, and biophysical techniques; including recombinant protein production, x-ray crystallography, surface plasmon resonance, enzymatic assays, flow cytometry, and mass spectrometry. As of 2021, I am an Australian Research Council DECRA Fellow, with my research focusing on CD1-lipid antigen presentation and mediation in autoreactivity.
Research interests
Our group within the Rossjohn laboratory focuses on the role of lipids in human T-cell mediated immune responses. Lipid antigens are presented by MHC class I like proteins, denoted as CD1 molecules, on the surface of specialised antigen presenting cells, to receptors on T cells (TCRs). CD1 molecules are generally grouped into two groups: Group 1 (CD1a, CD1b, CD1c), and Group 2 (CD1d), based on sequence similarities, intracellular trafficking pathways, and antigen binding groove architecture. Group 2 CD1 molecules have been the most well characterised molecules in the context of lipid antigen presentation to invariant Natural Killer T cells, however little is known on the mechanisms of Group 1 CD1 molecules. Our group focuses on characterising the molecular mechanisms of Group 1 CD1 molecule lipid antigen presentation, and subsequent TCR recognition. Group 1 CD1 molecules have been found to play a role in bacterial infection, namely by Mycobacterium tuberculosis, and autoimmunity, such as in skin hypersensitivities, psoriasis, cellular stress, and cancer. Using biophysical techniques and structural biology, namely x-ray crystallography, we aim to a) elucidate the mechanisms of self- and foreign- lipid antigen presentation by CD1a, CD1b, and CD1c, b) characterise the molecular mechanisms of TCR recognition, and c) determine their roles in the context of bacterial and autoimmune diseases. Our focus will not only provide a deeper understanding of the role of lipid antigens in the context of human T-cell mediated immunity, but will provide a molecular basis for the development of novel therapeutics against targeted diseases.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Network
Projects
- 1 Active
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Defining the structural basis of lipid mediated T cell immunity
Australian Research Council (ARC)
1/01/21 → 31/12/23
Project: Research
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Staphylococcal phosphatidylglycerol antigens activate human T cells via CD1a
Monnot, G. C., Wegrecki, M., Cheng, T-Y., Chen, Y. L., Sallee, B. N., Chakravarthy, R., Karantza, I. M., Tin, S. Y., Khaleel, A. E., Monga, I., Uwakwe, L. N., Tillman, A., Cheng, B., Youssef, S., Ng, S. W., Shahine, A., Garcia-Vilas, J. A., Uhlemann, A. C., Bordone, L. A., Han, A., & 5 others , Jan 2023, In: Nature Immunology. 24, 1, p. 110-122 13 p.Research output: Contribution to journal › Article › Research › peer-review
1 Citation (Scopus) -
Novel molecular insights into human lipid-mediated t cell immunity
Shahine, A., Wegrecki, M. & Nours, J. L., 1 Mar 2021, In: International Journal of Molecular Sciences. 22, 5, 15 p., 2617.Research output: Contribution to journal › Review Article › Research › peer-review
Open Access3 Citations (Scopus) -
Rational design of a hydrolysis-resistant mycobacterial phosphoglycolipid antigen presented by CD1c to T cells
Reijneveld, J. F., Marino, L., Cao, T-P., Cheng, T. Y., Dam, D., Shahine, A., Witte, M. D., Filippov, D. V., Suliman, S., van der Marel, G. A., Moody, D. B., Minnaard, A. J., Rossjohn, J., Codée, J. D. C. & van Rhijn, I., Oct 2021, In: Journal of Biological Chemistry. 279, 4, 13 p., 101197.Research output: Contribution to journal › Article › Research › peer-review
Open Access3 Citations (Scopus) -
A single-domain bispecific antibody targeting CD1d and the NKT T-cell receptor induces a potent antitumor response
Lameris, R., Shahine, A., Pellicci, D. G., Uldrich, A. P., Gras, S., Le Nours, J., Groen, R. W. J., Vree, J., Reddiex, S. J. J., Quiñones-Parra, S. M., Richardson, S. K., Howell, A. R., Zweegman, S., Godfrey, D. I., de Gruijl, T. D., Rossjohn, J. & van der Vliet, H. J., Nov 2020, In: Nature Cancer. 1, 11, p. 1054-1065 12 p.Research output: Contribution to journal › Article › Research › peer-review
15 Citations (Scopus) -
Human γδ T cells recognize CD1b by two distinct mechanisms
Reijneveld, J. F., Ocampo, T. A., Shahine, A., Gully, B. S., Vantourout, P., Hayday, A. C., Rossjohn, J., Moody, D. B. & van Rhijn, I., 15 Sep 2020, In: Proceedings of the National Academy of Sciences of the United States of America. 117, 37, p. 22944-22952 9 p.Research output: Contribution to journal › Article › Research › peer-review
21 Citations (Scopus)
Prizes
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IUCr Young Scientist Travel Award
Shahine, Adam (Recipient), 2013
Prize: Prize (including medals and awards)
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Monash University MBio 3 Minute Thesis Competition
Shahine, Adam (Recipient), Aug 2012
Prize: Prize (including medals and awards)
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Monash University MBio 3 Minute Thesis Competition
Shahine, Adam (Recipient), Jul 2013
Prize: Prize (including medals and awards)
Activities
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BCH/IMM3990 Research in Action student supervision
Adam Shahine (Supervisor)
2019 → 2021Activity: Other Teaching Engagements and non-HDR Supervisions › Other
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Visiting Academic to Cardiff University, UK
Adam Shahine (Fellow)
Sep 2018 → Dec 2018Activity: External Academic Engagement › External research organisation, centre or institute
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Day of Immunology tour guide
Adam Shahine (Contributor)
12 May 2017Activity: Community Talks, Presentations, Exhibitions and Events › Public event
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CD1-MR1 2017
Adam Shahine (Speaker)
4 Nov 2017Activity: Participating in or organising an event types › Contribution to conference
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Roeland Lameris
Adam Shahine (Host) & Jamie Rossjohn (Host)
2 Feb 2017 → 18 Jun 2017Activity: Hosting a visitor types › Hosting an academic or industry visitor
Press/Media
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Novel immune-oncology approach for potential cancer treatment
15/09/20
1 Media contribution
Press/Media: Expert Comment
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Paper: A molecule with more to say in autoimmune diseases
Adam Shahine, Jamie Rossjohn & D Branch Moody
23/10/17
1 Media contribution
Press/Media: Expert Comment
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The skinny on lipid immunology
Adam Shahine & D Branch Moody
20/10/17
1 Media contribution
Press/Media: Expert Comment