20102024

Research activity per year

Personal profile

Biography

Dr Shahine received a BSc (2008) from the University of Melbourne, before moving to Monash University to complete an M.BSc(part 1) (2009) and PhD (2016) under the supervision of A/Prof Travis Beddoe and Prof. Jamie Rossjohn. Their postgraduate research into the structural and functional characterisation of novel enzymes in Mycobacterium tuberculosis provided a solid biochemical skillset. Upon completion, Dr Shahine has been employed by The Department of Biochemistry and Molecular Biology, Monash University, since 2014. Here, Dr Shahine research has focused on the field of structural immunology, where they aim to understand the critical events in lipid-mediated immunity in the context of autoreactivity and bacterial infection.

Through Dr Shahine's research, they employ a complementary set of biochemical, cellular, and biophysical techniques; including recombinant protein production, x-ray crystallography, surface plasmon resonance, enzymatic assays, flow cytometry, and mass spectrometry.

Dr Shahine was previously funded by an Australian Research Council DECRA Fellowship (2021-2023), and is currently an National Health and Medical Research Council Emerging Leader Fellow (2024-2028). Since 2023, Dr Shahine is a Group Leader within the Immunity Program at the Biomedicine Discovery Institute, and leads the lipid mediated structural immunology lab. 

Biography

Our research lab focuses on the role of lipids in human T-cell mediated immune responses. Lipid antigens are presented by MHC class I like proteins, denoted as CD1 molecules, on the surface of specialised antigen presenting cells, to receptors on T cells (TCRs). CD1 molecules are generally grouped into two groups: Group 1 (CD1a, CD1b, CD1c), and Group 2 (CD1d), based on sequence similarities, intracellular trafficking pathways, and antigen binding groove architecture. Group 2 CD1 molecules have been the most well characterised molecules in the context of lipid antigen presentation to invariant Natural Killer T cells, however little is known on the mechanisms of Group 1 CD1 molecules.

Our group focuses on characterising the molecular mechanisms of Group 1 CD1 molecule lipid antigen presentation, and subsequent TCR recognition. Group 1 CD1 molecules have been found to play a role in bacterial infection, namely by Mycobacterium tuberculosis, and autoimmunity, such as in skin hypersensitivities, psoriasis, cellular stress, and cancer.

Using biophysical techniques and structural biology, namely x-ray crystallography, we aim to a) elucidate the mechanisms of self- and foreign- lipid antigen presentation by CD1a, CD1b, and CD1c, b) characterise the molecular mechanisms of TCR recognition, and c) determine their roles in the context of bacterial and autoimmune diseases.

Our focus will not only provide a deeper understanding of the role of lipid antigens in the context of human T-cell mediated immunity, but will provide a molecular basis for the development of novel therapeutics against targeted diseases.

Expertise related to UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):

  • SDG 3 - Good Health and Well-being

Education/Academic qualification

Biochemistry, PhD, Structural Characterization of Novel Proteins from Mycobacterium species implicated in survivability, MONASH UNIVERSITY

20102016

Award Date: 21 Jul 2016

Biochemistry, Masters of Biomedical Sciences (Part 1), A Structural Investigation of the Tetrahydrolipstatin Drug Target, Rv3802c, from M. tuberculosis, MONASH UNIVERSITY

2009

Biochemistry, Microbiology, Biotechnology, BSc, University of Melbourne

20062008

Research area keywords

  • adaptive immunity
  • Mycobacterium tuberculosis

Collaborations and top research areas from the last five years

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