University College London

  • Hooi, Y. T. (Visiting researcher)
  • Soon Hao Tan (Visiting researcher)

Activity: Visiting an external institution types › Visiting an external academic institution

Description

Visited University College London and shadowed the researchers from The Molecular NeuroPathobiology Laboratory in performing the following techniques and procedures:

Animal work
• Live imaging of endosomal signaling endosomes (sciatic nerve - live monitoring)
• Fluorescent tagging of the transport probe (e.g.: fragment of tetanus toxin - HcT)
• Dissection of sciatic nerve and muscles (tibialis anterior, soleus, extensor digitorum longus, gastrocnemius, lumbrical and flexor digitorum brevis) from mice.

In vitro work
• Harvesting embryo spinal cord and brains for neuronal culture
• Differentiation of neurons from human engineered iPS cells (i3N and i3LMN)
• Fabrication and set up of microfluidic chambers
• Live imaging of neuronal cells using confocal microscopy
• Labelling of TeNT HcT441 with Alexa Fluor
• Axonal retrograde transport assay - Test the axonal transport of organelles (e.g., HcT, mitochondria) within the different compartments of microfluidic chambers.

Period5 Sept 2022 → 30 Sept 2022
VisitingUniversity College London
Degree of RecognitionInternational

Keywords

  • Molecular Biology
  • Neuroscience